Vegetative propagation of trifoliate orange (Poncirus trifoliate)‎ via tissue culture technique

Abstract EN

The influence of the nutrient composition of plant tissue culture media on axillary shoot proliferation from shoot tips of orange trifoliate (Poncirus trifoliata) was investigated.

In recent years, the use of this rootstock for fruit trees has been increased dramatically.

Shoot tips were cultured on the following media: Murashige and Skoog (MS) (full, half and quarter strength) and Lloyd and McCown (WPM).

Media were supplemented with 2-benzyladenine (BA) in combinations with mg.l-1 Kintien and mg.l-1 GA3.

The media type and half strength of the media significantly affected the multiplication number, lengths (cm) of axillary shoots and number of leaves.

The WPM media and WPM containing half strength of mineral salts was the best.

Highest number of shoots per explant, maximum shoot length and highest leaves number (4.2, 5.18 cm and 13) (4.8, 6 cm and 13), respectively were obtained.