Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique
Other Title(s)
عزل و تشخيص جرثومة Salmonella typhimurium و الكشف عن الجين المشفر لتخليق النوع الهدبي الأول باستعمال تقنية تفاعلات سلسلة البلمرة (PCR)
Joint Authors
Muzhir, Balsam Miri
Jawad, Muhammad Sabri
al-Hasnawi, Zaynah Fuad Salih
Klaif, Siba Falah
Fahd, Khulud Hamdan
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 33, Issue 2 (31 Dec. 2019), pp.195-199, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
- Qadisiyah(City)
- Bacteria
- Genes
- Fimbriae(Microbiology)
- Polymerase chain reaction
- Cows
- Salmonella typhimurium
Abstract EN
Two hundred faeces sample were collected from cattle with different age and sex in Al- Diwaniyah Province.
The study was conducted in the period between November 2016 and November 2017.
Salmonella typhimurium bacteria identified by routine methods such as culturing on selective media, biochemical test and agglutination test using monovalent and multivalent antisera.
PCR was can detection type-1 fimbriae gene coding for fimC of Salmonella typhimurium.
Results showed that Salmonella isolates were 14.5% in the bovine fecal samples.
Also, the serotyping of isolates by using monovalent and polyvalent antisera revealed that all Salmonella isolates in cows were S.
typhimurium.
The PCR technique was used for detection of type-1 fimbriae coding gene by specific primer for fimC gene.
All S.
typhimurium isolates in cows appeared to be contained this gene show one distinct band MW.289 bp when electrophoresed on agarose gel.
The results of this score indicated that the PCR technique potentate a loud specify in the disclosing of S.
typhimurium especially the serotype that encoded to fimC gene type-1 fimbriae isolated from cows in comparison to other routine diagnostic tests.
American Psychological Association (APA)
al-Hasnawi, Zaynah Fuad Salih& Muzhir, Balsam Miri& Fahd, Khulud Hamdan& Jawad, Muhammad Sabri& Klaif, Siba Falah. 2019. Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique. Iraqi Journal of Veterinary Sciences،Vol. 33, no. 2, pp.195-199.
https://search.emarefa.net/detail/BIM-917079
Modern Language Association (MLA)
al-Hasnawi, Zaynah Fuad Salih…[et al.]. Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique. Iraqi Journal of Veterinary Sciences Vol. 33, no. 2 (2019), pp.195-199.
https://search.emarefa.net/detail/BIM-917079
American Medical Association (AMA)
al-Hasnawi, Zaynah Fuad Salih& Muzhir, Balsam Miri& Fahd, Khulud Hamdan& Jawad, Muhammad Sabri& Klaif, Siba Falah. Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique. Iraqi Journal of Veterinary Sciences. 2019. Vol. 33, no. 2, pp.195-199.
https://search.emarefa.net/detail/BIM-917079
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 198-199
Record ID
BIM-917079