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Molecular identification of allelic genotypes of pyrethroid-insecticide resistance in housefly, Iraq
Other Title(s)
التحديد الجزيئي للأليل المورث لمقاومة الحشرات للمبيدات الحشرية من نوع Pyrethroid في ذبابة المنزل، العراق
Joint Authors
al-Khalidi, Mansur Jadan Ali
al-Fatlawi, Munir Abd al-Amir Abd
Nasir, Hasan Hajim
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 33, Issue 2 (31 Dec. 2019), pp.209-212, 4 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
According to global-wide presence of insecticides resistance to pyrethroids, the current study identified the purpose to detect the allelic genotypes regarding this issue in house flies in Iraq.
From the governorate of Al-Qadisiyah, Iraq, 60 morphologically and molecularly recognized house flies were caught from 6 different regions.
Using a technique called polymerase chain reaction (PCR) amplification of specific allele (PASA), PCR was employed to reveal the presence of allelegenetic variations in the para-type sodium channel (para) gene to recognize knockdown resistance (kdr) mutation from the homozygous-wild type of complete susceptibility (sus/sus) to the mutated-homozygous type of complete resistance (kdr/kdr) or to the mutated-heterozygous type (kdr/sus).
Here, these genotypes were targeted using specific primers to identify these genetic variations.
The results have declared the presence of the sus/sus at 100%-frequency rate in all flies, and none of the other genotypes were detected (0%) in all flies.
This valued piece of result indicates the reality of resistance persistence due to lack of insecticide-spraying programs in the governorate.
This study provides high-quality information about the current status of insecticide resistance in house flies in Iraq about supporting the fact of genetic-base development of such resistance via frequent use of insecticides
American Psychological Association (APA)
al-Fatlawi, Munir Abd al-Amir Abd& al-Khalidi, Mansur Jadan Ali& Nasir, Hasan Hajim. 2019. Molecular identification of allelic genotypes of pyrethroid-insecticide resistance in housefly, Iraq. Iraqi Journal of Veterinary Sciences،Vol. 33, no. 2, pp.209-212.
https://search.emarefa.net/detail/BIM-917094
Modern Language Association (MLA)
al-Fatlawi, Munir Abd al-Amir Abd…[et al.]. Molecular identification of allelic genotypes of pyrethroid-insecticide resistance in housefly, Iraq. Iraqi Journal of Veterinary Sciences Vol. 33, no. 2 (2019), pp.209-212.
https://search.emarefa.net/detail/BIM-917094
American Medical Association (AMA)
al-Fatlawi, Munir Abd al-Amir Abd& al-Khalidi, Mansur Jadan Ali& Nasir, Hasan Hajim. Molecular identification of allelic genotypes of pyrethroid-insecticide resistance in housefly, Iraq. Iraqi Journal of Veterinary Sciences. 2019. Vol. 33, no. 2, pp.209-212.
https://search.emarefa.net/detail/BIM-917094
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 211-212
Record ID
BIM-917094