مشاركة الحمض الأميني البرولين مع جزيئات LDL في ممددات السائل المنوي عند كباش العواس

Abstract EN

The aim of this study was to evaluate the efficiency of combination amino acid proline with Low-Density Lipoproteins (LDL) into local semen extender for Awassi rams.

Semen was collected three times per week during the breeding season by the means of artificial vagina from 4 three-year Awassi rams with body weight 75±3 KG.

Semen was frozen in liquid nitrogen -196 C0 in five extenders: AndroMed (control), sodium citrate-glucose with 20% egg yolk (v/v) and proline at two concentrations 25 and 50 mM (EY+25P, EY+50P), sodium citrate-glucose with 8% LDL (w/v) and proline at two concentrations 25 and 50 mM (LDL+25 P, LDL+50 P).

Motility and live-dead sperms were evaluated at each step of semen handling by using phase contrast microscope and eosin-nigrosin staining technique for differential live - dead spermatozoa .

CASA system was used to evaluate the frozen semen motility parameters.

The percentages of live sperms e^imated under eosin-nigrosin staining technique were 66.40, 62.10, 60.70, 58.50 and 56.71 % in LDL+25P, LDL+50P, AndroMed®, EY+25P, and EY+50P respectively.

The extender containing 8% LDL and 25mM proline was significantly superior (P<0.001) to other experimental extenders.

The results of semen analysis by CASA system showed that there were significant differences (P<0.001) in the studied extenders.

Motility was 64.97, 60.30, 59.02, 56.40 and 53.51% in LDL+25P, LDL+50P, AndroMed®, EY+25P, and EY+50P respectively.

The progressive motility was 47.18, 44.86, 45.37, 42.14, and 39.85% respectively.

There was a significant difference among extenders (P<0.001) in term of VCL 90.8, 81.49, 82.57, 85.19, and 83.88um/s respectively.

The conclusion from this study is that the combination of amino acid proline with LDL fractions are synergistic, they have a cryoprotective effect and significantly enhanced motility parameters of frozen-thawed Awassi ram semen.