Activity of peroxidase in free radicals, human blood cells and effect of the PH and temperature on peroxidase activity and stability
Other Title(s)
فعالية انزيم البيروكسيديز في كبح الجذور العرة، خلايا دم الإنسان و تأثير الاس الهيدروجيني و درجة الحرارة في فعالية و استقرار الأنزيم
Joint Authors
Ali, Karrar Riyad Muhammad
Ali, Intisar Husayn
Source
Journal of University of Babylon for Engineering Sciences
Issue
Vol. 26, Issue 9 (30 Sep. 2018), pp.108-115, 8 p.
Publisher
Publication Date
2018-09-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
The previously studies of enzymes were focused only on the extraction and purification of enzyme and increase the toxic effect of drugs and supplement of free radical scavenging.
so, the aim of the study was to extend purified peroxidase from pseudomonas aeruginosa application and to abundant new enzymatic drug of free radicals scavenging with low side effect.
peroxidase is categorized as Oxido-reductases, which were used for catalyzing various oxidative reactions that protect cells from the harm effect of free radicals .peroxidase was tested as antioxidant and test their toxicity on human cells to overcome the problem of the side effect of drug and supplement that used as an antioxidant.
the enzyme was applied as h2o2 scavenging activity antioxidant by used three concentration of enzyme and triplicate (10, 40, 60 μg/ml).
the enzyme was show higher activity at 60 μg/ml reached to 40% activity.
the cytotoxicity of peroxidase was tested on whole healthy human blood by C.B.C by using equal amount 5μg of (enzyme substrate, only substrate and enzyme without substrate).
the result showed that the enzyme only had no effect on human blood as compare with h2o2 and enzyme with h2o2.
the characterization of enzyme was done by using more than one temperature and ph value and the result were shown that the optimal temperature for enzyme activity and stability rang (40-45°c) and the optimal ph for enzyme stability and activity were 7.
American Psychological Association (APA)
Ali, Karrar Riyad Muhammad& Ali, Intisar Husayn. 2018. Activity of peroxidase in free radicals, human blood cells and effect of the PH and temperature on peroxidase activity and stability. Journal of University of Babylon for Engineering Sciences،Vol. 26, no. 9, pp.108-115.
https://search.emarefa.net/detail/BIM-918542
Modern Language Association (MLA)
Ali, Karrar Riyad Muhammad& Ali, Intisar Husayn. Activity of peroxidase in free radicals, human blood cells and effect of the PH and temperature on peroxidase activity and stability. Journal of University of Babylon for Engineering Sciences Vol. 26, no. 9 (2018), pp.108-115.
https://search.emarefa.net/detail/BIM-918542
American Medical Association (AMA)
Ali, Karrar Riyad Muhammad& Ali, Intisar Husayn. Activity of peroxidase in free radicals, human blood cells and effect of the PH and temperature on peroxidase activity and stability. Journal of University of Babylon for Engineering Sciences. 2018. Vol. 26, no. 9, pp.108-115.
https://search.emarefa.net/detail/BIM-918542
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 113-114
Record ID
BIM-918542