Isolation and screening of Streptomyces sp. Al-Dhabi-49 from the environment of Saudi Arabia with concomitant production of lipase and protease in submerged fermentation
Joint Authors
Arasu, Mariadhas Valan
Ismail, Jalal Ali
Ghilan, Abdal-Karim Muhammad
al-Dhabi, Nayif Abd Allah
Source
Saudi Journal of Biological Sciences
Issue
Vol. 27, Issue 1 (31 Jan. 2020), pp.474-479, 6 p.
Publisher
Publication Date
2020-01-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
In this study, Streptomyces sp.
Al-Dhabi-49 was isolated from the soil sample of Saudi Arabian environment for the simultaneous production of lipase and protease in submerged fermentation.
The process parameters were optimized to enhance enzymes production.
The production of protease and lipase was found to be maximum after 5 days of incubation (139.2 ± 2.1 U/ml, 253 ± 4.4 U/ml).
Proteolytic enzyme increases with the increase in pH up to 9.0 (147.2 ± 3.6 U/ml) and enzyme production depleted significantly at higher pH values.
In the case of lipase, production was maximum in the culture medium containing pH 8.0 (166 ± 1.3 U/ml).
The maximum production of protease was observed at 40 °C (174 ± 12.1 U/ml) by Streptomyces sp.
Lipase activity was found to be optimum at the range of temperatures (30–50 °C) and maximum production was achieved at 35 °C (168 ± 7.8 U/ml).
Among the evaluated carbon sources, maltose significantly influenced on protease production (218 ± 12.8 U/ml).
Lipase production was maximum when Streptomyces sp.
was cultured in the presence of glucose (162 ± 10.8U/ml).
Among various concentrations of peptone, 1.0% (w/v) significantly enhanced protease production.
The lipase production was very high in the culture medium containing malt extract as nitrogen source (86 ± 10.2 U/ml).
Protease production was maximum in the presence of Ca2+ as ionic source (212 ± 3.8 U/ml) and lipase production was enhanced by the addition of Mg2+ with the fermentation medium (163.7 ± 6.2 U/ml).
American Psychological Association (APA)
al-Dhabi, Nayif Abd Allah& Ismail, Jalal Ali& Ghilan, Abdal-Karim Muhammad& Arasu, Mariadhas Valan. 2020. Isolation and screening of Streptomyces sp. Al-Dhabi-49 from the environment of Saudi Arabia with concomitant production of lipase and protease in submerged fermentation. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.474-479.
https://search.emarefa.net/detail/BIM-920746
Modern Language Association (MLA)
al-Dhabi, Nayif Abd Allah…[et al.]. Isolation and screening of Streptomyces sp. Al-Dhabi-49 from the environment of Saudi Arabia with concomitant production of lipase and protease in submerged fermentation. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.474-479.
https://search.emarefa.net/detail/BIM-920746
American Medical Association (AMA)
al-Dhabi, Nayif Abd Allah& Ismail, Jalal Ali& Ghilan, Abdal-Karim Muhammad& Arasu, Mariadhas Valan. Isolation and screening of Streptomyces sp. Al-Dhabi-49 from the environment of Saudi Arabia with concomitant production of lipase and protease in submerged fermentation. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.474-479.
https://search.emarefa.net/detail/BIM-920746
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 478-479
Record ID
BIM-920746