Molecular identification of Todiramphus chloris subspecies on the Arabian Peninsula using three mitochondrial barcoding genes and ISSR markers
Joint Authors
Shobrak, Muhammad
Boland, Christopher
Pereira, John
Budd, Jane
Hasan, Muhammad M.
Jabir, Ahmad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 27, Issue 1 (31 Jan. 2020), pp.480-488, 9 p.
Publisher
Publication Date
2020-01-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
The Collared Kingfisher (Todiramphus chloris) is widely distributed across the Indian and western Pacific Oceans and consists of about 50 subspecies.
Two different subspecies of T.
chloris occur in the Arabian Peninsula: T.
c.
abyssinicus from the Red Sea coast and T.
c.
kalbaensis from the Arabian Sea coast in the United Arab Emirates and Oman.
The aim of this study was to determine the molecular relationship between the two Arabian subspecies and to establish the first DNA barcodes from the Arabian Peninsula for this species.
Three different mitochondrial genes were used: (i) cytochrome c oxidase subunit I (COI), (ii) 12S rRNA (12S) and (iii) NADH dehydrogenase-1 (ND1).
The COI gene sequences of the two subspecies were 100% identical, while the 12S and ND1 gene sequences revealed a unique single nucleotide variation between the two subspecies.
Thus, this single nucleotide variation can be used as a DNA barcode to discriminate between two subspecies.
Furthermore, the genetic profile or fingerprint for both subspecies were compared using ten primers of the highly polymorphic nuclear markers (Inter Simple Sequence Repeat, ISSR).
As expected, the DNA analysis of the ISSR markers was able to distinguish between the specimens of the two subspecies.
These results suggest that T.
c.
abyssinicus and T.
c.
kalbaensis are not identical and thus belong to different subspecies.
Besides, the sequences of the COI gene for T.
c.
abyssinicus and T.
c.
kalbaensis differs by only 1.28% from T.
sanctus suggesting that the Arabian subspecies are closely related to the Sacred Kingfisher (T.
sanctus).
American Psychological Association (APA)
Jabir, Ahmad& Hasan, Muhammad M.& Boland, Christopher& Pereira, John& Budd, Jane& Shobrak, Muhammad. 2020. Molecular identification of Todiramphus chloris subspecies on the Arabian Peninsula using three mitochondrial barcoding genes and ISSR markers. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.480-488.
https://search.emarefa.net/detail/BIM-920752
Modern Language Association (MLA)
Jabir, Ahmad…[et al.]. Molecular identification of Todiramphus chloris subspecies on the Arabian Peninsula using three mitochondrial barcoding genes and ISSR markers. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.480-488.
https://search.emarefa.net/detail/BIM-920752
American Medical Association (AMA)
Jabir, Ahmad& Hasan, Muhammad M.& Boland, Christopher& Pereira, John& Budd, Jane& Shobrak, Muhammad. Molecular identification of Todiramphus chloris subspecies on the Arabian Peninsula using three mitochondrial barcoding genes and ISSR markers. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.480-488.
https://search.emarefa.net/detail/BIM-920752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 487-488
Record ID
BIM-920752