Determination of doxycycline hyclate in pharmaceutical samples via oxidative coupling reaction using spectrophotometric method

Abstract EN

This study involves the development of spectrophotometric method for determination of microgram a suggested mounts of doxycycline Hyclate (DCH) via oxidative coupling with 2,4,dinitrophenylhydrazine reagent (2,4-DNPH) in presence of potassium periodate(KIO4) in the alkaline medium (pH 12.6) to form a red colored dye which soluble in water and gives the highest intensity of absorption at λmax 528 nm.

The method has been obeyed Beer's law in the concentration range 5-60 μg.ml-1with the molar absorptivity of 10771 L.

mol-1.

cm-1, sandel index value 0.0476 μg.cm-2 with the relative standard deviation of the method does not exceed its value 1.043 % and the correlation coefficient 0.998.

the proposed method was applied successfully for the determination of doxycycline hyclate in its pharmaceutical samples (Capsule).