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Sequencing of gliotoxin genes in clinical and environmental Aspergillus fumigatus isolates in Iraq
Other Title(s)
تتابع الحمض النووي لـ جينات Gliotoxin في فطر Aspergillus fumigatus المعزولة من مصادر سريرية وبيئية في العراق
Joint Authors
Abd al-Hadi, Rusul Salih
Abd al-Baqi, Nimat Jamil
Source
Journal of Biotechnology Research Center
Issue
Vol. 12, Issue 1 (30 Jun. 2018), pp.33-39, 7 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Gliotoxin is an important virulence factors in Aspergillus fumigatus.
The biosynthesis of this mycotoxin is regulated and expressed by the presence of gliP genes.
This study aimed to identify Aspergillus fumigatus isolates in clinical and environmental sources with glip genes using conventional PCR and sequence.
To achieve this, DNA was isolated from twenty A.
fumigatus isolates using commercial kit.
The range of the DNA extracted was 65-210 ng/μl with a purity of 1.5-1.9.
Species identification of the A.
fumigatus isolates was achieved to a high specificity by using tailored primer.
The results showed that all isolates had positive results to the primer and all isolates were able to produce gliotoxin.
PCR detected the gliotoxin genes, glip in five isolates.
The five PCR product samples were sent for sequence analysis and 25 μl (10 pmol) from the forward primer.
The results of all the samples indicated have a single band of the desired product of gliP gene of A.fumigatus and the samples sent for sequencing related to molecular weight 190 bp.
American Psychological Association (APA)
Abd al-Hadi, Rusul Salih& Abd al-Baqi, Nimat Jamil. 2018. Sequencing of gliotoxin genes in clinical and environmental Aspergillus fumigatus isolates in Iraq. Journal of Biotechnology Research Center،Vol. 12, no. 1, pp.33-39.
https://search.emarefa.net/detail/BIM-921320
Modern Language Association (MLA)
Abd al-Hadi, Rusul Salih& Abd al-Baqi, Nimat Jamil. Sequencing of gliotoxin genes in clinical and environmental Aspergillus fumigatus isolates in Iraq. Journal of Biotechnology Research Center Vol. 12, no. 1 (2018), pp.33-39.
https://search.emarefa.net/detail/BIM-921320
American Medical Association (AMA)
Abd al-Hadi, Rusul Salih& Abd al-Baqi, Nimat Jamil. Sequencing of gliotoxin genes in clinical and environmental Aspergillus fumigatus isolates in Iraq. Journal of Biotechnology Research Center. 2018. Vol. 12, no. 1, pp.33-39.
https://search.emarefa.net/detail/BIM-921320
Data Type
Journal Articles
Language
English
Record ID
BIM-921320