Determination of combining ability and genetic diversity using ISSR markers to evaluate the genetic variability in wheat

Other Title(s)

تحديد القدرة على التألف و التباعد الوراثي باستخدام تكنيك ISSR لتقييم التنوع الوراثي في القمح

Joint Authors

al-Sadun, Ammar Wabdan
Sidhum, Sidhum Asad
al-Badawi, Mahmud al- Zabalawi
al-Husari, Ahmad Ali Abd al-Maqsud
al-Husari, Ahmad Ali Abd al-Maqsud

Source

مجلة تكريت للعلوم الزراعية

Publisher

Tikrit University College of Agriculture

Publication Date

2018-12-31

Country of Publication

Iraq

No. of Pages

14

Main Subjects

Botany

Topics

• Plant genetics
• Biological diversity
• Agriculture
• Wheat
• Growth

English Abstract

Eight bread wheat genotypes were screened by the six ISSR primers and scored a total of 54 amplified DNA bands.

The number of bands varied from 40 to 69 bands form primers ISSR 2 (6 fragments) to ISSR 5 (13 fragment).

Number of polymorphic fragments varied from 1 for ISSR 4 to 8 for ISSR 1 and ISSR 5.

The six ISSR primers generated 314 scorable bands across 8 studied genotypes.

These primers produced a total of 54 reproducible fragments, from which 30 (55.55%) were polymorphic.

The lowest genetic similarity (0.63) was obtained between P3 and P7, while, the highest genetic similarity (0.89) was scored between P7 and P8.

The parents were crossed in a 8x8 half diallel scheme in 2015/2016.

Parents and their 28 F1 crosses were evaluated under normal and stress conditions during 2016/2017 in two irrigation levels experiments.

The mean squares were significant for all studied traits.

The highest mean values were detected by parents P2, P2, P8, P2, P6 and p2 for plant height, spike length, no of spike/ plant, 1000-kernel weight, biological yield/ plant and grain yield/ plant in the combined analysis, respectively.

While, the highest mean values were recorded under combined analysis with crosses P1xP6 for biological yield/ plant and the cross P2xP4 for grain yield / plant.

Mean squares for combining ability estimates were highly significant for all studied traits.

The ratios GCA /SCA exceeded the unity for all studied traits, revealing that additive and additive x additive types of gene action are more important than non-additive gene action in controlling these traits.

The parental P6 exhibited positive and significant ĝi effects for grain yield/ plant and its attribute.

The highest desirable SCA effects were obtained with the crosses P1xP6, P1xP7, P2xP4, P3xP7, P3xP8, P4xP8, P5xP8 and P6xP7 for grain yield/ plant which exhibited significant and positive ŝij effects.

Positive correlation coefficients were found between genetic diversity and each of mean performance and SCA for grain yield/plant.

Hence, ISSR marker can be used as a tool for determining the extent of genetic diversity among wheat genotypes and can be used to precisely predict the yield performance value for F1 hybrids.

Data Type

Conference Papers

Record ID

BIM-922152

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