Molecular identification and biological resistance to local isolate of the tomato mosaic virus (ToMV)‎ on the sweet pepper plants

Abstract EN

The study aimed to identification the ToMV virus in some Salah aldin governorate fields from locally infected plants using RT-PCR technique, which gave clear bande at 318 bp and 512bp.

It also the study including the use of Spirulina platensis and Ganoderma lucidium, and their interaction (Ga.

+S.P.).

Their effect on stimulating the resistance against ToMV virus and some characteristics of growth also studied.

The treatment showed that S.platensis and G.lucidum with highest rate in stimulating the production of peroxidase enzyme at 4.8 and 4.6 unit/ ml respectively, while for the treatment without infection it was 2.2 unit / ml for both treatments.

The intraction treatment (Ga +S.P.) increased percentage of the fruiting and dry weight was (85.4%, and 80.97 g respectively, compared to control treatment (62% and 39.35 g respectively), while the treatment with S.platensis gave an increase in the height of pepper plants reached to 22.2 cm compared to control treatment 16.8 cm.