Molecular detection of erm (A), mef (A) in Staphylococcus spp and Streptococcus spp resistant to macrolide from different clinical infections
Other Title(s)
الكشف الجزيئي للجين erm (A) و mef (A) في Staphylococcus spp. و Streptococcus spp المقاومة لمضادات المايكرولايد والمعزولة من مصادر سريرية مختلفة
Author
Source
Diyala Agricultural Sciences Journal
Issue
Vol. 9, Issue (s) (31 Dec. 2017), pp.1-15, 15 p.
Publisher
University of Diyala College of Agriculture
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Abstract EN
Isolates Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus albus, Streptococcus Pyogenes and Streptococcus agalactiae isolated from different clinical infections were activated.
Results of susceptibility test showed that Staphylococcus aureus have resistance to antibiotics of erythromycin, azithromycin and clindomycin with percentage 45%, 45% 25% respectively, while Staphylococcus epidermidis resistant to erythromycin, azithromycin and clindomycin with ratio 66.6%, 44%, 22.2%, respectively.
The resistance of erythromycin and azithromycin by Streptococcus pyogenes reached 25% and 37.5% respectively, whereas all isolates sensitive to clindomycin.
The outcome disclosed that the values of minimum inhibitory concentrations of erythromycin ranged between 32 - ≥ 64 µg/ mL for all isolates.
The results outcomes to 13(65%) isolates of Staphylococcus aureus produced Staphylokinase, 6(66.6%) isolates of Staphylococcus epidermidis able to produce the same enzyme.
The results proved that 3(37.5%) isolates of Streptococcus pyogenes able to produce Streptokinase enzyme, but Streptococcus agalactiae could not produce streptokinase.
The PCR-based assay, gene was detected in erm (A) gene was detected in 33.3% isolate of Staphylococcus spp.
and 50% of Streptococcus spp, whereas mef (A) was detected in 46.6 % isolates of Staphylococcus spp.
and 50% of Streptococcus spp .These findings demonstrated the variable phenotypic expression when compared to genotypic detection .
American Psychological Association (APA)
al-Tai, Hadi Rahman Rashid. 2017. Molecular detection of erm (A), mef (A) in Staphylococcus spp and Streptococcus spp resistant to macrolide from different clinical infections. Diyala Agricultural Sciences Journal،Vol. 9, no. (s), pp.1-15.
https://search.emarefa.net/detail/BIM-922471
Modern Language Association (MLA)
al-Tai, Hadi Rahman Rashid. Molecular detection of erm (A), mef (A) in Staphylococcus spp and Streptococcus spp resistant to macrolide from different clinical infections. Diyala Agricultural Sciences Journal Vol. 9, Special issue (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-922471
American Medical Association (AMA)
al-Tai, Hadi Rahman Rashid. Molecular detection of erm (A), mef (A) in Staphylococcus spp and Streptococcus spp resistant to macrolide from different clinical infections. Diyala Agricultural Sciences Journal. 2017. Vol. 9, no. (s), pp.1-15.
https://search.emarefa.net/detail/BIM-922471
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-922471