تقويم كفاءة أربع طرائق مختلفة لاستخلاص الحمض النووي الريبي المنزوع الأوكسيجين (DNA)‎ من سوسة القمح Sitophilus granaries

Abstract EN

Molecular studies are important tools in the classification of insects, and the extraction of DNA from insects is the first step in such studies.

The aim of the present study was to evaluate four methods of DNA extraction from the insect Sitophilus granarius, and comparing the yield and purity of the extracted DNA by the various methods.

The results showed that the SDS (sodium dodecyl sulfate) method produced the highest DNA yield but with low purity.

CTAB-PVP (ctyltrimethylammonium bromide polyvinyl-pyrrolidone) and CTAB (ctyltrimethylammonium bromide) were appropriate methods of DNA extraction in terms of yield and purity, but the DNA yield was lower than that produced by the DTT (dithiotreiotol) method.

In addition, the DTT method produced reasonable purity of the extracted DNA from S.

granariues and maintained the shape and structure of the insect.