Effect of Myostatin gene polymorphisms on some qualitative characteristics of carcass and ifferent body measurements for broiler chicken (Ross 308)‎ raised under of Iraq condition

Abstract EN

Three hundreds of Ross 308 chicks at one day-old were used.

The experiment was continuous until sixth week of age.

The objective of this study was to identify genotypes Myostatin gene and its relation with some qualitative characteristics of carcass and different body measurements for broiler chicken.

Three types of restriction enzymes Aci I (Arthrobacter citreus), Bbv I (Bacillus brevis) and Bbs I (Bacillus brevis), were used in the Restriction fragment length polymorphism (RFLP) analysis.

The results of this study can be summarized as follows: The percentages of genotypic frequency for the Myostatin gene by using restriction enzyme Aci I (Arthrobacter citreus) was 32 %, 54% and 14 % for the (GG, GA and AA), respectively.

The differences between genotypes was highly significant, furthermore, the effect of the genotypes of the Myostatin gene on carcass yield percentage was significant (P<0.05) too .Effect of the genotypes of the Myostatin gene on the body measurement was significant (P<0.05) on the breast width during third week and the body length during sixth week.

The percentage of genotype distribution for the Myostatin gene by restriction enzyme Bbs I was 6.78%, 86% and 31.36 % for the genotypes.

(CC, CT and TT), respectively and the differences between these percentages was highly significant and the effect of the genotypes of the Myostatin gene on the body measurement was significant (P<0.05) on the leg length during third week and leg length, the body length and comb height during sixth week.

The percentage of genotype distribution for the Myostatin gene by restriction enzyme Bbv I in samples of studied was 98% and 2 % for the genotypes, (AA and GA) respectively and the differences between these percentages was highly significant and the effect of the genotypes of the Myostatin gene on the body measurement was significant (P<0.01) in the leg length and thigh thickness during sixth week.