The siRNA efficacy of soluble acid invertase down-regulation in sugarcane (Saccharum SPP.)‎

Abstract EN

Sugarcane (Saccharum sp.

hybrids) is a C4 grass used as a major source of sucrose.

Invertase enzymes hydrolyse sucrose into hexose sugars reducing the production markedly.

Soluble acid invertase role is always a case of discussion for having a major or minor role in the breakdown pro-cess in sink tissues.

Bio-deterioration is another serious problem accomplishes the sucrose produc-tion, the delay between harvest and milling of sug-arcane cause enormous depreciation in cane ton-nage as well as sugar recovery.

Beside another many factors, it was improved that both neutral and acid invertase present in cane stalk and both have tendency to increase after harvest.

In the present study, sugarcane cultivar G.99/103, Sac-charum officinarium, was used to establish In-planta transformation experiment for down-regula-tion of soluble acid invertase gene using siRNA application.

The transgenic plants were examined chemically and genetically to estimate the percent-age of silencing and its impact on the sucrose con-tent.

The enzyme activity showed reduction com-pared to control in most transgenic plants and con-sequently the decrease in expression level of solu-ble acid invertase increase the Brix value signifi-cantly in some of the transgenic plants.