دراسة ارتياديه في المختبر لتقييم فعالية معالجات كيميائية و توليفية في القضاء على الخلايا الجذعية السرطانية الثديية المتهمة بنكس الورم

Abstract EN

Background & Objectives: A plethora of research studies during the last decade assured the role of cancer stem cells (CSCs) in tumorogenesis, resistance to traditional chemotherapies, and tumor relapse.

New research in this field aims at determining optimal drug combinations to target these cells and eradicate tumors.

Methods: Cells were isolated from primary breast cancer samples, then cultured in-vitro in adherent and non-adherent conditions, followed by measuring expression of several breast cancer specific genes before and after exposing cultured cells to traditional and non-traditional chemotherapies prescribed to breast cancer patients.

Results: Some isolated cells maintained the characteristics of breast cancer stem cells (BCSCs) as for their slow doubling time in culture, mammosphere formation in non-adherent conditions, and increased expression of specific genes.

Additionally, cells responded differentially to applied drugs, with the highest cytotoxicity attributed to Doxorubicin, Ebetaxel, and Simvastatin.

Conclusion: This research offers a laboratory model to screen traditional and non-traditional drugs and drug combinations targeting breast cancer stem and non-stem cells, paving the way to support the most effective therapeutic decision successful in tumor eradication.