Influence of explant and growth regulators in the induction of callus of annona muricata in vitro

Abstract EN

his study was conducted in the laboratory of plant tissue culture Department of Horticulture and Landscape , College of Agriculture – University of Baghdad during 2015-2016.

The study includ using of plant tissue culture technology in the induction of callus from young explants (Leaf and node) planted in the MS media containing different concentrations of growth regulators, with the use of different auxins (Picloram, NAA and Cytokinin BA) at different concentrations for the purpose of induction callus.

The results showed, that the young leaf was excelled on the young node which was planted in the MS media with a concentration of (1.2 mg.L-1) picloram and (0.6 mg.L-1) BA , the highest fresh and dry weights of callus were obtained reached of (212.25, 22.43 mg) respectively, compared to the young node which gave, (94.00, 11.43 mg) at the same concentration of fresh and dry weight, respectively.

The young leaf was excelled on the young nods for the of Annona muricata in the MS media at a concentration of (1.0 mg.L-1) NAA and (0.6 mg.L-1) BA, which it gave the highest fresh and dry weights of the callus (185.00, 21.43 mg), respectively, compared to young nods which gave, (140.43, 14.86 mg) at the same concentration, of fresh and dry weight of callus respectively.