Evaluation of proteolytic bacillus spp. isolated from soil and characteristion of their growth and activity of proteases
Other Title(s)
تقييم بكتريا الباسليس المحللة للبروتين والمعزولة من التربة وخصائص نموها و نشاط انزيمات البروتيازيس
Joint Authors
al-Wafai, Nahid Amin
Salim, Samir Hammad
al-Zabalawi, Hind Muhammad Abd al-Sadiq
Mahjub, Samir Ahmad Margani
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 44, Issue 6 (31 Dec. 2017)17 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The aim of this study was to isolate, screen and optimize proteolytic bacteria from different soil samples.
Soil samples collected from El-Sharkia Governorate and Wadi Abu Sobeira, Aswan Governorate were screened for proteolytic bacteria.
The later soil was characterized by saline soil.
Proteolytic bacteria from each soil was studied by cultivating in a protease production medium (PPM), low in carbon content.
Substrate specificity of the isolated proteolytic bacteria was assayed by adding seven nitrogen sources and six carbon sources.
Each one was added to PPM compared to the control (peptone and glucose), respectively.
Four bacterial isolates were selected and they exhibited excellent ability to hydrolyze high-molecular weight proteins (skim milk, gelatin and casein).
These isolates showed extracellular protease activity on skim milk agar plates (20 mm) after 24 hr., at 37P◦PC.
These isolates were identified as Bacillus cereus strain A1(4), B.
toyonensis A3(14), B.
cereus strain A4(26) and B.
cereus strain A8(60) based on partial sequencing of 16S rDNA.
Protease production by these strains [A1 (4), A3 (14), A4 (26) and A8(60)] compared to B.
subtilis cultivate in PPM reached a maximum at 24 and 48 hr., with values ranged between 81.83 and 76.16 U/ml.
Starch and mannitol were the best substrates for enzyme production, while glycerol could not influence production of protease.
Among various organic nitrogen sources, beef extract, soybean and skim milk, which are, commercial, were found as the best substrate for bacterial growth and enzyme production, while NaNOR3R, KNOR3R and (NH4)R2RSOR4R could not affect production of protease or bacterial growth.
This enzyme showed high capability for removing proteins and stain spots from cloths.
American Psychological Association (APA)
al-Zabalawi, Hind Muhammad Abd al-Sadiq& al-Wafai, Nahid Amin& Mahjub, Samir Ahmad Margani& Salim, Samir Hammad. 2017. Evaluation of proteolytic bacillus spp. isolated from soil and characteristion of their growth and activity of proteases. Zagazig Journal for Agricultural Researches،Vol. 44, no. 6.
https://search.emarefa.net/detail/BIM-932323
Modern Language Association (MLA)
al-Zabalawi, Hind Muhammad Abd al-Sadiq…[et al.]. Evaluation of proteolytic bacillus spp. isolated from soil and characteristion of their growth and activity of proteases. Zagazig Journal for Agricultural Researches Vol. 44, no. 6 (2017).
https://search.emarefa.net/detail/BIM-932323
American Medical Association (AMA)
al-Zabalawi, Hind Muhammad Abd al-Sadiq& al-Wafai, Nahid Amin& Mahjub, Samir Ahmad Margani& Salim, Samir Hammad. Evaluation of proteolytic bacillus spp. isolated from soil and characteristion of their growth and activity of proteases. Zagazig Journal for Agricultural Researches. 2017. Vol. 44, no. 6.
https://search.emarefa.net/detail/BIM-932323
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-932323