Hepatoprotective effects of Moringa Oleifera extracts on acetaminophen-induced oxidative damage in rats

Abstract EN

This study aimed to evaluate the in-vitro antiox-idant activities of Moringa Oleifera Lam (MO) ex-tracts, and their protective effects in acetamino-phen (APAP)- induced liver injury in rats caused by oxidative damage.

The antioxidants activities of ethanolic, aqueous and hexane extracts of differ-ent MO edible parts were investigated by DPPH radical scavenging capacity and malondialdehyde (MDA) assays.

The ethanolic extracts of different parts of MO were found to have higher antioxidant capacity compared to the aqueous and hexane extracts.

The flowers ethanolic extract has the highest total antioxidants capacity among the other different parts of MO follo wed by leaves, pods, roots and finally seeds.

HPLC-MS scanning of eth-anolic leave extracts showed the presence of fla-vonoid derivates Apigenin, quercetin and kaempferol in addition to chlorogenic acid.

In the hepatoprotective study, either leaves or pods ex-tracts (300mg / Kg bw or 600mg / Kg bw stomach tube orally) were administrated to rats one hour prior to administration of a single dose of APAP (4g / Kg bw by stomach tube orally).

The hepatopro-tective activity of MO leaves and pods extracts were followed for 21 days by observed in the levels of liver markers such as alanine aminotransferase (ALT) and the levels of oxidative damage markers including superoxide dismutase (SOD) and malondialdehyde (MDA) and catalase (CAT), wich analysed and compared between groups pretreat-ed with MO extracts +APAP to those treated with APAP alone.

The outcome of this parameters indi-cate reduction in the severity of liver damage in group treated with MO extracts + APAP and com-pared to those treated with APAP alone .

Also, histopathological examination of liver tissues of rats treated with MO extracts showed an improve-ment at the end of experiment.

The results of this study indicate the hepatoprotective properties of MO leaves and pods ethanolic extracts against liver injury and thereby signify its traditional use.