Quantitative determination of fluoroquinolones in contaminated soils by HPLC with solid-phase extraction
Other Title(s)
التقدير الكمي للفلوروكوينولونات في التربة الملوثة بواسطة كروماتوغرافيا السائل العالي الأداء مع الاستخلاص بالطور الصلب
Joint Authors
Muhammad, Alya Abd al-Razzaq
Kamil, Ghadah Mahdi
Motelica Heino, Mikael
Morabito, Domenico
Qasim, Bashshar Husayn
Source
Issue
Vol. 17, Issue 1 (31 Mar. 2020), pp.48-56, 9 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2020-03-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
This work reports the development of an analytical method for the simultaneous analysis of three fluoroquinolones; ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) in soil matrix.
The proposed method was performed by using microwave-assisted extraction (MAE), solid-phase extraction (SPE) for samples purification, and finally the pre-concentrated samples were analyzed by HPLC detector.
In this study, various organic solvents were tested to extract the test compounds, and the extraction performance was evaluated by testing various parameters including extraction solvent, solvent volume, extraction time, temperature and number of the extraction cycles.
The current method showed a good linearity over the concentration ranging from 1 – 300ng g-1 with correlation coefficients (r2 > 0.998) for all test compounds.
Good recoveries were also obtained for the test compounds ranged from 89 – 99 %.
The relative standard deviation (RSD) from intra-day and inter-day precision was lower than 7%, and the detection (LOD) and quantification (LOQ) limits ranged from 0.9 – 2.7ng g-1.Finally, the proposed method was applied to determine the target compounds in real soil samples irrigated with wastewater.
The results confirm the presence of the test compounds in soils with values ranging from 1.9 – 4.6ng g-1, as well as the suitability of the proposed method to determine the test compounds in real soil matrix as well as the ability of the test compounds to accumulate in soils irrigated with sewage.
American Psychological Association (APA)
Qasim, Bashshar Husayn& Muhammad, Alya Abd al-Razzaq& Kamil, Ghadah Mahdi& Motelica Heino, Mikael& Morabito, Domenico. 2020. Quantitative determination of fluoroquinolones in contaminated soils by HPLC with solid-phase extraction. Baghdad Science Journal،Vol. 17, no. 1, pp.48-56.
https://search.emarefa.net/detail/BIM-935364
Modern Language Association (MLA)
Qasim, Bashshar Husayn…[et al.]. Quantitative determination of fluoroquinolones in contaminated soils by HPLC with solid-phase extraction. Baghdad Science Journal Vol. 17, no. 1 (2020), pp.48-56.
https://search.emarefa.net/detail/BIM-935364
American Medical Association (AMA)
Qasim, Bashshar Husayn& Muhammad, Alya Abd al-Razzaq& Kamil, Ghadah Mahdi& Motelica Heino, Mikael& Morabito, Domenico. Quantitative determination of fluoroquinolones in contaminated soils by HPLC with solid-phase extraction. Baghdad Science Journal. 2020. Vol. 17, no. 1, pp.48-56.
https://search.emarefa.net/detail/BIM-935364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 55-56
Record ID
BIM-935364