Indirect spectrofluorometric method for the determination of cefotaxime sodium, ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate-bromide and acriflavine dye
Other Title(s)
طريقة فلورومترية غير مباشرة لتقدير السيفوتاكسيم صوديوم و هيدروكلوريد السيبروفلوكساسين و الفاموتيدين باستخدام برومات-بروميد و صبغة الأكريفلافين
Joint Authors
Said, Abd al-Samad Muhammad Ali
Salih, Ilham Sad Allah
Source
Issue
Vol. 17, Issue 1 (31 Mar. 2020), pp.66-77, 12 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2020-03-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
An Indirect simple sensitive and applicable spectrofluorometric method has been developed for the determination of Cefotaxime Sodium (CEF), ciprofloxacin Hydrochloride (CIP) and Famotidine (FAM) using reaction system bromate-bromide and acriflavine (AF) as fluorescent dye.
The method is based on the oxidation of drugs with known excess bromate-bromide mixture in acidic medium and subsequent determination of unreacted oxidant by quenching fluorescence of AF.
Fluorescence intensity of residual AF was measured at 528 nm after excitation at 402 nm.
The fluorescence-concentration plots were rectilinear over the ranges 0.1-3.0, 0.05-2.6 and 0.1-3.8 µg ml-1 with lower detection limits of 0.013, 0.018 and 0.021 µg ml-1 and quantitation limits of 0.044, 0.060 and 0.069 µg ml-1 for CEF, CIP and FAM respectively.
The common excipients and additives didn’t interfere in their determination.
The developed method was successfully applied for determination of the studied drugs in their dosage forms resulted in a good agreement with standard British pharmacopeia method and standard addition procedure.
American Psychological Association (APA)
Said, Abd al-Samad Muhammad Ali& Salih, Ilham Sad Allah. 2020. Indirect spectrofluorometric method for the determination of cefotaxime sodium, ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate-bromide and acriflavine dye. Baghdad Science Journal،Vol. 17, no. 1, pp.66-77.
https://search.emarefa.net/detail/BIM-935372
Modern Language Association (MLA)
Said, Abd al-Samad Muhammad Ali& Salih, Ilham Sad Allah. Indirect spectrofluorometric method for the determination of cefotaxime sodium, ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate-bromide and acriflavine dye. Baghdad Science Journal Vol. 17, no. 1 (2020), pp.66-77.
https://search.emarefa.net/detail/BIM-935372
American Medical Association (AMA)
Said, Abd al-Samad Muhammad Ali& Salih, Ilham Sad Allah. Indirect spectrofluorometric method for the determination of cefotaxime sodium, ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate-bromide and acriflavine dye. Baghdad Science Journal. 2020. Vol. 17, no. 1, pp.66-77.
https://search.emarefa.net/detail/BIM-935372
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 65-67
Record ID
BIM-935372