Molecular identification of Fusobacterium isolates and limitation of biofilm formation adhesion gene (fadA)‎ in dental outpatients

Abstract EN

Fusobacterium are compulsory anaerobic gram-negative bacteria, long thin with pointed ends, it causes several illnesses to humans like pocket lesion gingivitis and periodontal disease; therefore our study is constructed on molecular identification and detection of the fadA gene which is responsible for bacterial biofilm formation.

In this study, 10.2 % Fusobacterium spp.

were isolated from pocket lesion gingivitis.

The isolates underwent identification depending on several tests under anaerobic conditions and biochemical reactions.

All isolates were sensitive to Imipenem (IPM10) 42.7mm/disk, Ciprofloxacin (CIP10) 27.2mm/disk and Erythromycin (E15) 25mm / disk, respectively.

100 % of Fusobacterium spp.

isolates had 16S rDNA gene (360bp.), whereas two isolates had fadA gene (232bp.)