Analysis of MRP1 and MRP3 expression profile in liver and spleen of hyperbilirubinemic rodents
Other Title(s)
تحليل التعبير الجيني و البروتيني للنواقل MRP1 and MRP3 في أنسجه الكبد و الطحال للقوارض التي تعاني من ارتفاع مستويات البليروبين في الدم
Joint Authors
Qaysiah, Muhammad
Bellarosa, Cristina
Source
Hebron University Research Journal : Natural Sciences
Issue
Vol. 8, Issue - (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hebron University Deanship of Scientific Research
Publication Date
2019-12-31
Country of Publication
Palestine (West Bank)
No. of Pages
14
Main Subjects
Abstract EN
Unconjugated bilirubin (UCB) is produced mostly in the spleen from the turnover of heme.
UCB is conjugated by the activity of hepatic UGT1A1enzyme (UDP-glucuronosyltransferase 1A1) and exported into the bile.
High serum level of UCB is toxic to cells, and its intracellular accumulation is suggested to be limited by multidrug resistance proteins 1 and 3 (MRP1 and MRP3) that export UCB out of the cells to prevent its toxicity.
In this study, the expression profile for MRP1 and MRP3 was analysed by qRT-PCR and Western blot in the liver and the spleen of two animal models of hyperbilirubinemia (β-thalassemic mouse and Gunn rat).
The results indicated that MRP1 mRNA and protein expression remained unchanged in the liver and spleen of both animal models.
MRP3 mRNA and protein are up-regulated in the spleen of β-thalassemic mouse and liver of Gunn rat.
In conclusion, high level of UCB induces only the expression of MRP3 which may indicate its possible role in preventing UCB toxicity.
American Psychological Association (APA)
Qaysiah, Muhammad& Bellarosa, Cristina. 2019. Analysis of MRP1 and MRP3 expression profile in liver and spleen of hyperbilirubinemic rodents. Hebron University Research Journal : Natural Sciences،Vol. 8, no. -, pp.1-14.
https://search.emarefa.net/detail/BIM-936352
Modern Language Association (MLA)
Qaysiah, Muhammad& Bellarosa, Cristina. Analysis of MRP1 and MRP3 expression profile in liver and spleen of hyperbilirubinemic rodents. Hebron University Research Journal : Natural Sciences Vol. 8 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-936352
American Medical Association (AMA)
Qaysiah, Muhammad& Bellarosa, Cristina. Analysis of MRP1 and MRP3 expression profile in liver and spleen of hyperbilirubinemic rodents. Hebron University Research Journal : Natural Sciences. 2019. Vol. 8, no. -, pp.1-14.
https://search.emarefa.net/detail/BIM-936352
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 12-14
Record ID
BIM-936352