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Influence of yeast extract on gene expression of crwrky1 as a regulatory gene in alkaloids pathway in catharanthus roseus (l.) g. don
Other Title(s)
تأثير مستخلص الخميرة علي تعبير جين Crwrky1 كجين تنظيمي في مسار القلويدات Catharanthus roseus في الونكا
Joint Authors
Yunus, Raniya Ahmad Abd al-Maqsud
Maghazi, Muna Muhammad
Badawi, Fatimah Muhammad Ibrahim
Hassanin, al-Sayyid Hasan
Source
Arab Universities Journal of Agricultural Sciences
Issue
Vol. 26, Issue 2 (31 Dec. 2018), pp.647-654, 8 p.
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2018-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Catharanthus roseus (L.) G.
Don is a medicinal plant rich in many alkaloids that are used in treatment of many diseases.
It is unique in the production of vinblastine and vincristine compounds, which are used in treatment of several cancers.
There are many genes in the biosynthesis pathway that produce these anti-cancer compounds, including the gene Crwrky1 which is a regulatory gene that codes for the transcription factor in the monoterpene indole alkaloid (MIA) pathway.
In this study, the effect of yeast extract on crwrky1 gene expression levels from treated calli was studied.
Quantitative RT-PCR (QRT-PCR) using SYBR Green I/ROX was used to analyze the changes in the expression level of this gene in response to different treatments of yeast extract.
Crwrky1 expression increased to 3.6 folds in treated callus obtained under 0.4 mg/l yeast elicitor for 4 hours (YE2) treatment to compare with the control (untreated) callus
American Psychological Association (APA)
Maghazi, Muna Muhammad& Badawi, Fatimah Muhammad Ibrahim& Yunus, Raniya Ahmad Abd al-Maqsud& Hassanin, al-Sayyid Hasan. 2018. Influence of yeast extract on gene expression of crwrky1 as a regulatory gene in alkaloids pathway in catharanthus roseus (l.) g. don. Arab Universities Journal of Agricultural Sciences،Vol. 26, no. 2, pp.647-654.
https://search.emarefa.net/detail/BIM-939377
Modern Language Association (MLA)
Maghazi, Muna Muhammad…[et al.]. Influence of yeast extract on gene expression of crwrky1 as a regulatory gene in alkaloids pathway in catharanthus roseus (l.) g. don. Arab Universities Journal of Agricultural Sciences Vol. 26, no. 2 (2018), pp.647-654.
https://search.emarefa.net/detail/BIM-939377
American Medical Association (AMA)
Maghazi, Muna Muhammad& Badawi, Fatimah Muhammad Ibrahim& Yunus, Raniya Ahmad Abd al-Maqsud& Hassanin, al-Sayyid Hasan. Influence of yeast extract on gene expression of crwrky1 as a regulatory gene in alkaloids pathway in catharanthus roseus (l.) g. don. Arab Universities Journal of Agricultural Sciences. 2018. Vol. 26, no. 2, pp.647-654.
https://search.emarefa.net/detail/BIM-939377
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-939377