Application of salmonella phage cocktail to control salmonella typhimurium in vitro

Abstract EN

Members of the genus Salmonella are among the microbial pathogens associated with dangerous infection.

The increased of Salmonella spp.

antibiotic resistance has propelled the need of alternate therapeutic methods or strategies.

Bacteriophage forms one of these alternate strategies.

Six lytic bacteriophages infecting Salmonella Typhimurium were isolated from sewage drain water.

Phages were purified biologically by single plaque assay and concentrated using the ultracentrifugation.

The phage isolates were named Ø SM, Ø SF, Ø SG, Ø SP, Ø SA and Ø SD.

Morphological characters of Salmonella phages showed that all the phage isolates belong to family Myoviridae.

All phage isolates were highly stable at room temperature, storage at refrigerator temperature and had thermal inactivation point ranged from 90 to 98 °C.

Phages were stable at pH conditions ranging from pH 4 to 12.

Phages did not lose their infectivity after exposure to UV for 90 min.

at 35 cm and 53 cm distance.

The host range of the isolated phages was found that the phages had narrow host range.

Phage cocktail with different MOI was used to control Salmonella Typhimurium in vitro.

Data revealed that addition of phage cocktail at MOI 10 reduced the Salmonella cells with rate 98.2 % after 4 hrs.

from addition