Molecular detection of Torque Teno Virus (TTV)‎ infection of patients with haemoglobinopathies and haematological malignancies

Abstract EN

The existence of Torque teno virus (TTV) DNA in different organs indicates the presence of wide affinity to the host cell.

The present study aimed to detect TTV molecularly in the samples of haemoglobinopathies and haematological malignancies patients.

The results of semi-nested PCR showed a positive amplification in 2 % of serum samples of haemoglobinopathies patients, while no positive amplification was observed in 50 EDTA bloods samples of haematological malignancies patients.

One sample of positive cases was sequenced successively.

The sequence was compared with deposited copies of TTV in the NCBI using BLAST software and the results showed a sequence 96.64 % with KY750543.1.

The result of the nucleotide sequence analysis has shown mutations at TTV genome corresponding to ORF1.

The phylogenetic analysis results of our case ORF1 sequence with several selected ORF1 copies from NCBI displayed five main clades included grouping of our case ORF1 with the Italian (AJ402211.1) one in the same clade.

The result of the amino acids sequence analysis was produced one peptide (58 amino acids) that showed a similarity of 68.97 % with unnamed peptide (partial [Torque teno virus] Sequence ID: BAA74998.1 Length: 67) and a 51.72 % with ORF1 (partial [Torque teno virus] Sequence ID: AAL08949.1 Length: 84).

The present study concluded that the prevalence of TTV infections was remarkably low and it exhibits extensive genetic variation.