Detection ESBL enzymes in gram-negative bacteria
Other Title(s)
الكشف عن أنزيمات ESBL في البكتريا السالبة لصبغة كرام
Joint Authors
al-Hardani, Muna Jabbar Tumah
al-Imarah, Sad Shakir Mahdi
Makki, Hasanayn Muhammad Ali
al-Abd Allah, Amani Abd al-Rida
Source
Journal of Basrah Researches : Sciences
Issue
Vol. 45, Issue 2A (30 Jun. 2019), pp.53-59, 7 p.
Publisher
University of Basrah College of Education for Pure Sciences
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
The prevalence of Extended-spectrum ß-lactamase (ESBLs) enzymes are considered a large problem all over the world.
The misuse of broad-spectrum-antibiotics especially in immunocoprmized and hospitalized patients has lead to emergence of antibiotic-resistant strains of Enterobacteriaceae and several Gram-negative bacteria (GNB), for that reason, there's an urgent need to investigate these enzymes by a continuously examination and blood culture of these patients.
Blood samples n=147 were collected from Hemodialysis patients that treatment in Basrah General Teaching Hospital and Al-Sader Hospital in Basrah governorate south of Iraq, conventional microbiological methods (Biochemical tests) and also molecular methods by implified 16s rDNA and genetic sequence analysis, were used to dignosis the common types of Gram-negative bacteria and two methods were used, double disc synergy test (DDST) and double disc approximation method (DAM) to detect the present of ESBLs-enzymes in these samples.
Twenty seven of Gram-negative bacteria were obtained out of 147 blood samples, and out of 27 GNB, 16(59.3 %) was positive produced ESBLsenzymes.
Cefotaxime (CTX), was found to be the more sensitive to detect the ESBLsenzymes.
American Psychological Association (APA)
al-Hardani, Muna Jabbar Tumah& al-Abd Allah, Amani Abd al-Rida& al-Imarah, Sad Shakir Mahdi& Makki, Hasanayn Muhammad Ali. 2019. Detection ESBL enzymes in gram-negative bacteria. Journal of Basrah Researches : Sciences،Vol. 45, no. 2A, pp.53-59.
https://search.emarefa.net/detail/BIM-939548
Modern Language Association (MLA)
al-Hardani, Muna Jabbar Tumah…[et al.]. Detection ESBL enzymes in gram-negative bacteria. Journal of Basrah Researches : Sciences Vol. 45, no. 2A (2019), pp.53-59.
https://search.emarefa.net/detail/BIM-939548
American Medical Association (AMA)
al-Hardani, Muna Jabbar Tumah& al-Abd Allah, Amani Abd al-Rida& al-Imarah, Sad Shakir Mahdi& Makki, Hasanayn Muhammad Ali. Detection ESBL enzymes in gram-negative bacteria. Journal of Basrah Researches : Sciences. 2019. Vol. 45, no. 2A, pp.53-59.
https://search.emarefa.net/detail/BIM-939548
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-939548