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PCR based identification of Culex pipiens complex (Diptera : Culicidae) collected in Al-Najaf Governorate
Other Title(s)
التشخيص المعتمد على تقنية التفاعل التضاعفي لسلسلة الدنا PCRلمعقد بعوض الكيولكس (Diptera : Culicidae) في محافظة النجف الأشرف
Joint Authors
al-Rabii, Hadi Mazal
al-Husayni, Ma Allah Turki
Ali, Abd al-Zahrah Kazim Muhammad
Source
al-Kufa University Journal for Biology
Issue
Vol. 5, Issue 2 (31 Dec. 2013)12 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2013-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Total genomic DNA was extracted from the whole mosquitoes according to the manufacturer’s modified insect DNA extraction protocol.
The amplification of the acetylcholinesterase-2 (ace-2) locus by specific primer in a Polymerase Chain Reaction (PCR) and the flanking region of the CQ11 microsatellite methods were successfully employed to detect the genetic variation between Cx.
pipiens complex mosquitoes, PCR technique was applied on 200 individually adults Cx.
pipiens complex mosquitoes collected from underground and aboveground of breeding sites from Najaf governorate using diagnostic primer sets for Cx.
quinquefasciatus, Cx.
pipiens and Cx.
molestus.
The Agarose gel Electrophoresis results of PCR assay were revealed that Cx.
quinquefasciatus species was distributed at percentage reached 68% followed by Cx.
molestus at percentage 24% and 1.33 % for Cx.
pipiens.
Hybrids between Cx.
quinquefasciatus and Cx.
pipiens were 5.33% whereas hybrids between Cx.
pipiens and Cx.
molestus were limited.
One of the important results of the present study was that the molecular analysis and by this technique can be detected levels the degree of hybridization between close related species for Cx.
pipiens complex.
The results based on PCR revealed that Cx.
molestus species was most abundant than Cx.
pipiens and specimens of Cx.
molestus were obtained commonly from underground and rarely from aboveground.
American Psychological Association (APA)
al-Husayni, Ma Allah Turki& Ali, Abd al-Zahrah Kazim Muhammad& al-Rabii, Hadi Mazal. 2013. PCR based identification of Culex pipiens complex (Diptera : Culicidae) collected in Al-Najaf Governorate. al-Kufa University Journal for Biology،Vol. 5, no. 2.
https://search.emarefa.net/detail/BIM-939712
Modern Language Association (MLA)
al-Husayni, Ma Allah Turki…[et al.]. PCR based identification of Culex pipiens complex (Diptera : Culicidae) collected in Al-Najaf Governorate. al-Kufa University Journal for Biology Vol. 5, no. 2 (2013).
https://search.emarefa.net/detail/BIM-939712
American Medical Association (AMA)
al-Husayni, Ma Allah Turki& Ali, Abd al-Zahrah Kazim Muhammad& al-Rabii, Hadi Mazal. PCR based identification of Culex pipiens complex (Diptera : Culicidae) collected in Al-Najaf Governorate. al-Kufa University Journal for Biology. 2013. Vol. 5, no. 2.
https://search.emarefa.net/detail/BIM-939712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-939712