PCR detection of enterotoxins and methecillin resistances genes in staphylococcus aureus isolated from local food in Sulaimania City

Abstract EN

Present study was carried out to evaluate bacterial contaminated foods in local markets of Sulaimania city.

A total 250 samples were examined for isolation and identification of Staph.

aureus, that producer to enterotoxins and resistances to methicillin .

The samples included dressing of cake, soft chesses (white), ready to eat foods, red meat, and poultry.

The results appeared that: the incidence of Staphylococci were identified in 175 (70%) of the total samples from those; 104(42%) were positive for Staph.

aureus which had the ability to grow on the mannitol salt agar media, the most contaminate foods were dressing of cake 51% and soft chess 45% , While red meat was 44% and poultry 48% as well as ready to eat foods which contaminated with 35% of the samples.

According to biochemical testes; Staph.

aureus isolates had the ability to produce many enzymes like protease lipase, lethicinase , Coagulase , Catalase, also could fermented mannitol anaerobically and Hemolysin production, while all the isolates were oxidase negative.

The production of enterotoxin tested by culture methods for Staph.

aureus isolates showed that 57.14% of the isolates were enterotoxin producer.

The producer isolates were confirmed by PCR to detect the existence of (sea, seb and sec) genes, so 55%of the tested isolates possessed these genes.

Sensitivity to antibiotics applied to Staph.

aureus isolates that enterotoxin producer revealed different percentage of sensetivity to different antibiotics, the isolates appeared sensitivity toward pencillin, methicillin, vancomycin.

The Methicillin resistance Staph.

aureus which tested by disk diffusion methods was 81.6%, but by PCR was 73.3%.