Molecular detection of methicillin resistance in Staphylococcus aureus isolated from raw cow milk in Ghebaish Locality, West Kordofan
Joint Authors
Muhammad, G. E.
al-Hajj, Muna O.
Alseed, Alnazeer G.
Source
Sudan Journal of Science and Technology
Issue
Vol. 20, Issue 2 (31 Dec. 2019), pp.75-82, 8 p.
Publisher
Sudan University of Science and Technology Deanship of Scientific Research
Publication Date
2019-12-31
Country of Publication
Sudan
No. of Pages
8
Main Subjects
Abstract EN
The occurrence of MRSA organisms is one of the problems that facing the world now in treatment of bacterial infections.
The antibiotic resistance is common among pathogenic bacteria associated with both community acquired and nosocomial infections, Staphylococcus aureus is notorious for its ability to become resistant to antibiotics.
The aim of this study was to detect the specific mecA gene in the Staphyllococcus aureus isolates.
Staphylococci strains were isolated from raw cow milk in Ghebaish Locality and were identified with conventional methods and APi system.
A total of 200 samples were collected and 33 Staphylococci strains (16%) isolates were obtained.
Bacterial DNA was extracted from each isolate using boiling method.
PCR was used to detect mecA gene.
The results indicate that 23 isolates were positive to mecA gene (69%).The mecA gene sequence of S.
aureus showed high similarities with those recorded in gene bank.
The study concluded that, Bacterial DNA extracted from Staphylococcus aureus reflected appearance of mecA gene which was responsible of resistance to methicillin.
American Psychological Association (APA)
Alseed, Alnazeer G.& al-Hajj, Muna O.& Muhammad, G. E.. 2019. Molecular detection of methicillin resistance in Staphylococcus aureus isolated from raw cow milk in Ghebaish Locality, West Kordofan. Sudan Journal of Science and Technology،Vol. 20, no. 2, pp.75-82.
https://search.emarefa.net/detail/BIM-944538
Modern Language Association (MLA)
Alseed, Alnazeer G.…[et al.]. Molecular detection of methicillin resistance in Staphylococcus aureus isolated from raw cow milk in Ghebaish Locality, West Kordofan. Sudan Journal of Science and Technology Vol. 20, no. 2 (2019), pp.75-82.
https://search.emarefa.net/detail/BIM-944538
American Medical Association (AMA)
Alseed, Alnazeer G.& al-Hajj, Muna O.& Muhammad, G. E.. Molecular detection of methicillin resistance in Staphylococcus aureus isolated from raw cow milk in Ghebaish Locality, West Kordofan. Sudan Journal of Science and Technology. 2019. Vol. 20, no. 2, pp.75-82.
https://search.emarefa.net/detail/BIM-944538
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 80-82
Record ID
BIM-944538