Characterisation of ADAMs (protein) of bovine seminal plasma by mass spectrometry
Other Title(s)
وصيف ADAMs (بروتين) في البلازما المنوية البقري بواسطه تقنية المطياف الكتلي للبروتينات
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 10, Issue 2 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
This study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry.
The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract.
Seminal plasma of bovine was collected freshly and isolated after centrifuged and removed the sperm.
Non boiled and boiled seminal plasma lysate were run to identify and detect the total proteins, as well as the individual single member of ADAMs protein is determined.
The non-boiled lysate of seminal plasma was displayed diversity of proteins more than boiled sample lysate of seminal plasma.
However, boiled and non-boiled seminal plasma have distinguished several types of ADAMs protein which are included: ADAM10, ADAM9, ADAM7, ADAM15 in non-boiled samples, while boiled lysate of bovine seminal plasma was displayed ADAM10, ADAM9, ADAM28, and ADAM22.
Our finding concluded that bovine seminal plasma is very rich in different types of soluble, cleaved and shed proteins such ADAMs protein which could potentially have a biological and physiological role in protection and interactions of sperm during motility inside female reproductive tract, as it might support it to fertilise the ovum.
This protection might be via immunosuppression behaviour or by block specific receptors in female reproductive tract for enhancing sperm motility, and avoid sperm the singling response of immunity.
American Psychological Association (APA)
al-Muhanna, Hazim. 2019. Characterisation of ADAMs (protein) of bovine seminal plasma by mass spectrometry. Kufa Journal for Veterinary Medical Sciences،Vol. 10, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-945839
Modern Language Association (MLA)
al-Muhanna, Hazim. Characterisation of ADAMs (protein) of bovine seminal plasma by mass spectrometry. Kufa Journal for Veterinary Medical Sciences Vol. 10, no. 2 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-945839
American Medical Association (AMA)
al-Muhanna, Hazim. Characterisation of ADAMs (protein) of bovine seminal plasma by mass spectrometry. Kufa Journal for Veterinary Medical Sciences. 2019. Vol. 10, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-945839
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-7
Record ID
BIM-945839