Role of E4Orf3 protein in adenovirus life cycle in correlatin with E1B55k and promyelocytic leukemia protein Isoform II
Other Title(s)
دور بروتين اطار الشفرة المفتوحة المبكر في دورة حياة فيروس الحليمات الغدية خلال التأثير على البروتين الفيروسي المود للاورام و على بروتينات ما قبل النسيلة الصيغة الثانية الخلوية
Author
Source
Issue
Vol. 37, Issue 1 (30 Jun. 2019), pp.73-89, 17 p.
Publisher
University of Basrah College of Science
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
17
Main Subjects
Abstract EN
Promyelocytic leukemia protein isoform II (PML-II) mRNA expression was interfered with anti-PML-II- specific mRNA to downregulate its expression to analyse the infection outcome in presence or absence of PML-II.
PML-II transient knockdown gave an effective down regulation at the protein level then the transfected cells were challenged with adenovirus wild type (wt300) or Orf3- deleted mutant (InOrf3) to analyze the outcome of both viruses infection.
DNA binding protein (DBP) expression increased in both types of infection with the removal of PML-II with more or less level of infection in both types of infection.
Adenovirus yield was higher with the removal of PML-II in both types of wt or InOrf3 infection.
Interferon treatment reduced the expression of early protein ( E1b55k) in InOrf3 infection but not in wt infection when PML-II was absent.
To conclude, E4Orf3 removal reduces the virus yield at later time points of infection through effect on E1b55k in an interferon induced environment.
American Psychological Association (APA)
Atwan, Zaynah Wahid. 2019. Role of E4Orf3 protein in adenovirus life cycle in correlatin with E1B55k and promyelocytic leukemia protein Isoform II. Basrah Journal of Science،Vol. 37, no. 1, pp.73-89.
https://search.emarefa.net/detail/BIM-946682
Modern Language Association (MLA)
Atwan, Zaynah Wahid. Role of E4Orf3 protein in adenovirus life cycle in correlatin with E1B55k and promyelocytic leukemia protein Isoform II. Basrah Journal of Science Vol. 37, no. 1 (2019), pp.73-89.
https://search.emarefa.net/detail/BIM-946682
American Medical Association (AMA)
Atwan, Zaynah Wahid. Role of E4Orf3 protein in adenovirus life cycle in correlatin with E1B55k and promyelocytic leukemia protein Isoform II. Basrah Journal of Science. 2019. Vol. 37, no. 1, pp.73-89.
https://search.emarefa.net/detail/BIM-946682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 83-87
Record ID
BIM-946682