Bacteriological and molecular detection of Staphylococcus Aureussuperantigenic toxin genes in women ,cows and buffaloes milk
Other Title(s)
الكشف الجرثومي و الجزيئي لجرثومة Staphylococcus aureus و المستضدات الفائقة لذيفاناتھا في حليب النساء و الأبقار و الجاموس
Joint Authors
Mahdi, Aminah Shakir
Abd Allah, Fawziyah Ali
Source
Basrah Journal of Veterinary Research
Issue
Vol. 18, Issue 1 (30 Jun. 2019), pp.301-317, 17 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
17
Main Subjects
Topics
Abstract EN
A total of 300 random samples of milk represented by women, cows and buffaloes (100 for each) were examined by conventional bacteriology, serology and molecular biology tests for the presence of Staphylococcus aureus and its super antigenic toxin and its relation to methicillin-resistance.Positive bacterial isolation was observed in 82(27.3%.) milk samples,including37 (37%) cows, 30 (30%) women and 15 (15%) buffaloes samples.
By using latex agglutination test,all buffaloes milk S.
aureus isolatesexhibitedpositive results(100% ,n=15)followed by isolates of women(83.1%,n=25)and cows(81.1%,n=30) with an overall percentage(23.3%,n=70.).
Amplification of S aureus species, staphylococcal superantigenic toxin (sea, seb and sec) and methicillin-resistant S.
aureus(mecA) gene using Polymerase Chain Reaction (PCR) revealed that, 82/300 (27.3%) of the total examined milk samples were positive for bothspecies(nuc)and resistance(mecA) genes.
While, sea positive results exhibited by 5 (38.5)%) cows milkisolates .Both women and buffaloesmilk isolatesshowedsebpositive resultsin a percentage of 55.5% (5 isolates for each),sec was not producedby women milk isolates and appeared in cows and buffaloes milk isolates (7.7 and 11.1% respectively).Further confirmation for the S aureus diagnosis was performed by sequencing analysis and BLAST comparative analysis, the results of this analysis revealed that partial nucgene sequences of local tested isolates of S.
ureusshowed 100% sequence identity with published data of S.aureus(strain 08-02300, coStaphylococcusaureusmplete genome and Staphylococcus aureus isolate 22_LA_562 genome assembly, chromosome: I
American Psychological Association (APA)
Mahdi, Aminah Shakir& Abd Allah, Fawziyah Ali. 2019. Bacteriological and molecular detection of Staphylococcus Aureussuperantigenic toxin genes in women ,cows and buffaloes milk. Basrah Journal of Veterinary Research،Vol. 18, no. 1, pp.301-317.
https://search.emarefa.net/detail/BIM-946693
Modern Language Association (MLA)
Mahdi, Aminah Shakir& Abd Allah, Fawziyah Ali. Bacteriological and molecular detection of Staphylococcus Aureussuperantigenic toxin genes in women ,cows and buffaloes milk. Basrah Journal of Veterinary Research Vol. 18, no. 1 (2019), pp.301-317.
https://search.emarefa.net/detail/BIM-946693
American Medical Association (AMA)
Mahdi, Aminah Shakir& Abd Allah, Fawziyah Ali. Bacteriological and molecular detection of Staphylococcus Aureussuperantigenic toxin genes in women ,cows and buffaloes milk. Basrah Journal of Veterinary Research. 2019. Vol. 18, no. 1, pp.301-317.
https://search.emarefa.net/detail/BIM-946693
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 313-317
Record ID
BIM-946693