High performance liquid chromatographic method for determination of Tadalafil in tablets and wastewater

Abstract EN

Objective: A reverse phase high performance liquid chromatographic method (RPHPLC) has been developed for the determination of tadalafil in the pharmaceutical formulations and environmental wastewater samples.

Method: Different analytical columns with various stationary phases were tested.

Good separation was achieved using supelcoC18 column (25cm x 4.6 mm.

5 μm) .

The mobile phase (methanol: water: Triethylamine) , pH adjusted to 4.0 with dilute phosphoric acid was pumped at a flow rate of 1.3 ml/min in the ratio of 60:38:2 and the peaks were detected at 220 nm.

Results: Linearity was obtained in the concentration range of 0.0 4-0.28 mg/ml .The RSD was found to be less than 1% indicating the method is precise and the recovery was100±1.25% indicating the method is accurate Conclusion: The proposed HPLC method may be used for determining tadalafil in pure drug samples , pharmaceutical dosage forms and environmental wastewater samples.