Isolation and identification of lipA gene producing pseudomonas aeruginosa from industrial wastewater
Joint Authors
al-Diraghi, Wathiq Abbas
al-Hamadi, Farhan H.
Laftah, Intisar Taha
Source
Iraqi Journal of Biotechnology
Issue
Vol. 18, Issue 1 (30 Apr. 2019), pp.77-82, 6 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2019-04-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
Lipases are enzymes which have interesting in last few years for its widely applications in many industries like leather, detergents, cosmetic ,pharmaceuticals, biofuel, food, wastewater treatment etc.
Many organisms produce lipase like animals, plants, fungi and bacteria.
Pseudomonas aeruginosa lipases are very interesting to have some properties that are not common among lipases produced by other microorganisms, such as their thermoresistance and activity at alkaline pH ,that make it suitable to degrade oils in industrial wastewater.
This study aimed to isolation and identify for Pseudomonas aeruginosa from industerual wastewater (vegetable oils) depending on the polymerase chain reaction (PCR) targeted lipA gene.
Fifty samples were collected from oil rich industrial wastewater processed to routine biochemical tests to diagnosis P.
aeruginosa and compare these results with diagnosis by PCR technique based on gene lip A, it was found that Thirty four of fifty samples (68%) positive results with P.
aeruginosa .
Tow lipA gene primers were used to detect for P.
aeruginosa by the polymerase chain reaction Technique (PCR).
Throw tow primers used, lipA 948 was the best and more specialized primer to isolation P.
aeruginosa , (100%) positive result.
While the second primer lipA 558, (66.66%) positive result.
American Psychological Association (APA)
al-Diraghi, Wathiq Abbas& al-Hamadi, Farhan H.& Laftah, Intisar Taha. 2019. Isolation and identification of lipA gene producing pseudomonas aeruginosa from industrial wastewater. Iraqi Journal of Biotechnology،Vol. 18, no. 1, pp.77-82.
https://search.emarefa.net/detail/BIM-947591
Modern Language Association (MLA)
Laftah, Intisar Taha…[et al.]. Isolation and identification of lipA gene producing pseudomonas aeruginosa from industrial wastewater. Iraqi Journal of Biotechnology Vol. 18, no. 1 (Apr. 2019), pp.77-82.
https://search.emarefa.net/detail/BIM-947591
American Medical Association (AMA)
al-Diraghi, Wathiq Abbas& al-Hamadi, Farhan H.& Laftah, Intisar Taha. Isolation and identification of lipA gene producing pseudomonas aeruginosa from industrial wastewater. Iraqi Journal of Biotechnology. 2019. Vol. 18, no. 1, pp.77-82.
https://search.emarefa.net/detail/BIM-947591
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 81-82
Record ID
BIM-947591