Antibiotic susceptibility and molecular detection of pseudomonas aeruginosa isolated from bovine mastitis
Other Title(s)
الحساسية للمضادات الحياتية و التحديد الجزيئي لجرثومة الزوائف الزنجارية المعزولة من الأبقار المصابة بالتهاب الضرع
Joint Authors
al-Tai, Hala Said Rashid
al-Ahmad, Hazim Ismail Abd al-Bari
al-Samirrai, Ikram Abbas Abbud
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 43, Issue 2 (31 Dec. 2019), pp.77-85, 9 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
This study aimed to isolate Pseudomonas aeruginosa from cattle (bovine) milk with mastitis to characterize its antimicrobial susceptibility against some antibiotics, and to identify aminoglycoside acetyltransferase (aac-3-Ib) gene.A total of 100 bovine milk samples were collected randomly from different local cow farms at districts of Wasit governorate, Iraq.
Six P.
aeruginosa isolates were obtained using bacterial culture method and further identified by Analytical Profile Index (API-20E).
The antibiotic sensitivity test was performed by disc diffusion methods.
Among the 5 antibiotics used, the highest resistance (100%) was found with Nalidixic acid andtetracycline, follow by gentamicin (50%) and the lowest resistance rate (16.6%, and 33.3%) was to the ciprofloxacin and cephalothin respectively.
PCR was performed for all the gentamicin resistant isolates that be among the aminoglycoside family.
The where the frequency of aac(3)-Ib gene have product (530bp) was to 3 of P.
aeruginosa isolates.
From the finding of present study we concluded that P.aeruginosa thatisolated from mastitic bovinehave been developed resistance against aminoglycosides through presence of aac(3)-Ibgene and the ciprofloxacin and cephalothin can be taken as good choice of treatment.
American Psychological Association (APA)
al-Tai, Hala Said Rashid& al-Samirrai, Ikram Abbas Abbud& al-Ahmad, Hazim Ismail Abd al-Bari. 2019. Antibiotic susceptibility and molecular detection of pseudomonas aeruginosa isolated from bovine mastitis. Iraqi Journal of Veterinary Medicine،Vol. 43, no. 2, pp.77-85.
https://search.emarefa.net/detail/BIM-952754
Modern Language Association (MLA)
al-Tai, Hala Said Rashid…[et al.]. Antibiotic susceptibility and molecular detection of pseudomonas aeruginosa isolated from bovine mastitis. Iraqi Journal of Veterinary Medicine Vol. 43, no. 2 (2019), pp.77-85.
https://search.emarefa.net/detail/BIM-952754
American Medical Association (AMA)
al-Tai, Hala Said Rashid& al-Samirrai, Ikram Abbas Abbud& al-Ahmad, Hazim Ismail Abd al-Bari. Antibiotic susceptibility and molecular detection of pseudomonas aeruginosa isolated from bovine mastitis. Iraqi Journal of Veterinary Medicine. 2019. Vol. 43, no. 2, pp.77-85.
https://search.emarefa.net/detail/BIM-952754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 78-81
Record ID
BIM-952754