Isolation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane
Other Title(s)
عزل فايروس التهاب القصبات المعدي في الخلايا الابتدائية لغشاء اللقانقي المشيمي من أجنة البيض
Joint Authors
Zahid, Abd al-Amir Husayn Abd
Abd al-Ahad, Imad Adwar
Husayn, Mayadah Fuad
Muhammad, Majid Hamid
al-Azzawi, Amir
Hair-Bejo, M.
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 37, Issue 1 (31 Mar. 2013), pp.109-114, 6 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2013-03-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells.
Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR).
At 72 hours post-infection in third passage, the cytopathic effect was characterized by rounding up of cells, monolayer detachment, intracytoplasmic brownish colouration was readily observed by immunoperoxidase from 24hours p.i in third passage, and at all times the extracted viral RNA from IBV-infected monolayers was demonstrated by RT-PCR.
Tissue culture ineffective dose50 (TCID50) was used to measure virus titration performed on primary chick embryo chorioallontoic membrane cells and the titre in twenty passage was 108.6 TCID50/ml.The results obtained in this study suggested that the primary chick embryo chorioallontoic membrane cells can be used for adaptation infectious bronchitis virus (IBV) and may be considered a step forward for the use of these cells in the future for IBV vaccine production
American Psychological Association (APA)
Muhammad, Majid Hamid& Hair-Bejo, M.& Zahid, Abd al-Amir Husayn Abd& al-Azzawi, Amir& Abd al-Ahad, Imad Adwar& Husayn, Mayadah Fuad. 2013. Isolation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane. Iraqi Journal of Veterinary Medicine،Vol. 37, no. 1, pp.109-114.
https://search.emarefa.net/detail/BIM-955432
Modern Language Association (MLA)
Muhammad, Majid Hamid…[et al.]. Isolation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane. Iraqi Journal of Veterinary Medicine Vol. 37, no. 1 (2013), pp.109-114.
https://search.emarefa.net/detail/BIM-955432
American Medical Association (AMA)
Muhammad, Majid Hamid& Hair-Bejo, M.& Zahid, Abd al-Amir Husayn Abd& al-Azzawi, Amir& Abd al-Ahad, Imad Adwar& Husayn, Mayadah Fuad. Isolation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane. Iraqi Journal of Veterinary Medicine. 2013. Vol. 37, no. 1, pp.109-114.
https://search.emarefa.net/detail/BIM-955432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 113
Record ID
BIM-955432