Antibiofilm activity of chalcone in methicillin resistant Staphylococcus aureus
Joint Authors
al-Jabburi, Kamil M.
Tuthli, Khayriyah Jabir
al-Musawi, Intisar Taha
al-Mathkhuri, Harith Jabbar Fahd
Source
Iraqi Journal of Biotechnology
Issue
Vol. 18, Issue 3 (31 Dec. 2019), pp.62-71, 10 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
Methicillin-resistant Staphylococcus aureus (MRSA) is known as a key agent implicated in infections resulting in significant morbidity and mortality among patients.
MRSA can develop resistant structure named biofilm.
Among the various naturally occurring classes, chalcones are important intermediates for the biosynthesis of flavonoids.
Therefore, the current study aimed to investigate in vitro the inhibitory effect of chalcone on MRSA planktonic cells and biofilm.
Around 111 Staphylococcus aureus locally isolated from different clinical specimens were collected in this study.
Methicillin resistance was evaluated by detection mecA amplification by PCR assay.
Minimum inhibitory concentration (MIC) of chalcone was estimated by broth microdilution method.
Moreover, Atomic Force Microscopy (AFM) technique was employed for confirming the effect of chalcone on biofilm.
The present findings revealed that MRSA constituted, nearly, (93.22 %) (55 isolates) of all S.
aureus isolates.
Most of MRSA isolates were able to form biofilms, around 70.9% have the capacity to form strong biofilm.
Chalcone at MIC (5 µg/ml) significantly reduced the planktonic MRSA cells.
Whereas at Minimum Biofilm Inhibitory Concentration (MBIC) (20 µg/ml) and sub MBIC (15 µg/ml)reduced biofilm formation significantly and this findings are confirmed by using Atomic force microscopy.
In conclusion, suggest that chalcone could be a new agent for prevention of bacterial adherence to surfaces and could be identified as anew agent against S.
aureus infection.
American Psychological Association (APA)
al-Musawi, Intisar Taha& al-Jabburi, Kamil M.& al-Mathkhuri, Harith Jabbar Fahd& Tuthli, Khayriyah Jabir. 2019. Antibiofilm activity of chalcone in methicillin resistant Staphylococcus aureus. Iraqi Journal of Biotechnology،Vol. 18, no. 3, pp.62-71.
https://search.emarefa.net/detail/BIM-955676
Modern Language Association (MLA)
al-Musawi, Intisar Taha…[et al.]. Antibiofilm activity of chalcone in methicillin resistant Staphylococcus aureus. Iraqi Journal of Biotechnology Vol. 18, no. 3 (Dec. 2019), pp.62-71.
https://search.emarefa.net/detail/BIM-955676
American Medical Association (AMA)
al-Musawi, Intisar Taha& al-Jabburi, Kamil M.& al-Mathkhuri, Harith Jabbar Fahd& Tuthli, Khayriyah Jabir. Antibiofilm activity of chalcone in methicillin resistant Staphylococcus aureus. Iraqi Journal of Biotechnology. 2019. Vol. 18, no. 3, pp.62-71.
https://search.emarefa.net/detail/BIM-955676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 69-71
Record ID
BIM-955676