Increase the readiness of phosphorus to Rhaphanus sativus and Vigna unguiculata by local isolates of bacteria Pseudomonas spp

Abstract EN

In this research experiment, the action of the locally isolates of Pseudomonas spp., on the readiness and solubility of phosphorus (P) for plant was studied.

This study included two experiments, a laboratory and field once.

In the laboratory, diagnosis isolation of four isolates of Pseudomonas spp.(Ps1, Ps2, Ps3, and Ps4) from samples of the local Iraqi soil of different regions in Baghdad.

Through bio-fertilization, four isolates have been added to the soil for increasing the readiness of P to the plants field.

In the field experiment, Rhaphanus sativus and Vigna unguiculata were planted in plastic pots containing 5kg of soil and kept in a green house (Dept.

of biology /College of Sciences).

The soil in the pots were inoculated with broth of bacterial isolates.

Agriculture process was performed in March, and after 50 days the plants were harvested and the morphological characteristics were studied such as: plant length, fresh weight, and phosphorus concentration in plants and soil before and after the addition of bacterial isolates.

The results showed a significant increasing in growth parameters of plants, and in a percentage of P in plants and soils compared with the control plants.

All the isolates lead to increase the P percentage in plants, and soil, except the isolate Ps2 which was not affected , and did not show any significant result in this experiment.

Whereas, the isolate Ps4 was relatively the highest between the others in Vigna unguiculata , which the parameters of length, weight germination %, and P % were 31.27, 8.85, 90, and 5.37 respectively.

Although, Raphanus sativa parameters of length, weight, germination %, and P % were 21.53, 10.57, 80, and 5.98 respectively, the isolate Ps2 was the lowest among them for each of the plant radishes and Cowpeas.