Direct detection of 5ʹ-non coding region of all enterovirus serotypes genomes in stool samples
Other Title(s)
الكشف المباشر للمنطقة غير المشفرة بالطرف ʹ5 في جينوم كافة الأنماط المصلية للفايروس المعوي في عينات البراز
Joint Authors
al-Hamdani, Faysal Ghazi
Salih, Rafah Ali
Fadil, Hala Yunus
Abd al-Karim, Mais Jasim
Auff, Iman Mutashshar
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.151-161, 11 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
Cell culture of RD (Rhabdomyosaroma) and L20B (L-cell) are a gold test advised by the World Health Organization for enterovirus (EV) detection.
Otherwise, some EV serotypes no or slowly growing in common cell lines used for diagnosis, lack of cell sensitivity to low viral load in clinical samples, cost and long time required for final identification.
Hence, this study aimed to investigate conserved regions of all enterovirus serotypes genomes in the stool among acute flaccid paralysis (AFP) cases.
A total 68 samples were selected for RNA extraction directly from stool which were positive, weak positive, and negative results in cell culture among 130 stool samples, from children aged 5 months-7 years.
Reverse transcriptasePCR amplification relied on 5ʹnon-coding region (5ʹ-NCR) of EV genome with 144-149 bp nucleotide visualized on 1% agarose gel.
Results showed RT-PCR more accurate for positive cases (100%) with a short time diagnosis than cell culture, in addition to 3.85% detection of viral 5ʹ-NCR in negative sample.
Furthermore, 5ʹ-NCR RT-PCR is the best for overcoming cell culture problems and medical practice reduction that carry out AFP containment.
American Psychological Association (APA)
Abd al-Karim, Mais Jasim& Salih, Rafah Ali& Auff, Iman Mutashshar& al-Hamdani, Faysal Ghazi& Fadil, Hala Yunus. 2015. Direct detection of 5ʹ-non coding region of all enterovirus serotypes genomes in stool samples. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.151-161.
https://search.emarefa.net/detail/BIM-955938
Modern Language Association (MLA)
Fadil, Hala Yunus…[et al.]. Direct detection of 5ʹ-non coding region of all enterovirus serotypes genomes in stool samples. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.151-161.
https://search.emarefa.net/detail/BIM-955938
American Medical Association (AMA)
Abd al-Karim, Mais Jasim& Salih, Rafah Ali& Auff, Iman Mutashshar& al-Hamdani, Faysal Ghazi& Fadil, Hala Yunus. Direct detection of 5ʹ-non coding region of all enterovirus serotypes genomes in stool samples. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.151-161.
https://search.emarefa.net/detail/BIM-955938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 158-161
Record ID
BIM-955938