Direct detection of 5ʹ-non coding region of all enterovirus serotypes genomes in stool samples

Abstract EN

Cell culture of RD (Rhabdomyosaroma) and L20B (L-cell) are a gold test advised by the World Health Organization for enterovirus (EV) detection.

Otherwise, some EV serotypes no or slowly growing in common cell lines used for diagnosis, lack of cell sensitivity to low viral load in clinical samples, cost and long time required for final identification.

Hence, this study aimed to investigate conserved regions of all enterovirus serotypes genomes in the stool among acute flaccid paralysis (AFP) cases.

A total 68 samples were selected for RNA extraction directly from stool which were positive, weak positive, and negative results in cell culture among 130 stool samples, from children aged 5 months-7 years.

Reverse transcriptasePCR amplification relied on 5ʹnon-coding region (5ʹ-NCR) of EV genome with 144-149 bp nucleotide visualized on 1% agarose gel.

Results showed RT-PCR more accurate for positive cases (100%) with a short time diagnosis than cell culture, in addition to 3.85% detection of viral 5ʹ-NCR in negative sample.

Furthermore, 5ʹ-NCR RT-PCR is the best for overcoming cell culture problems and medical practice reduction that carry out AFP containment.