Adhesion capacity of Bifidobacterium to abiotic surfaces

Abstract EN

Bifidobacterium is a 13Tgenus13T of 13TGram-positive13T, 13Tnonmotile13T, often branched 13Tanaerobic13T 13Tbacteria13T.

They are ubiquitous, 13Tendosymbiotic13T inhabitants of the 13Tgastrointestinal tract13T, some bifidobacteria are used as 13Tprobiotics13T.The adhesion of Bifidobacterium on abiotic surfaces(stainless steel , polystyrene and galvaniz iron ) in cultures with different temperatures (5,37 and 40◦C) and different pHs (4, 7, and 9 ) were studied in recent study .This isolate gave positive results with congored agar and Christensen method .The results indicated that the type of material had effect on the attachment capacity of Bifidobacterium in other word , the galvaniz iron was significant highest attractive surface to bacterial cells and biofilm formation(P < 0.05) ,then follows by the stainless steel while polystyrene was the least .On other hand ,adhesion to all solid surfaces was influenced significantly by temperatures, the best adhesion occured at 40◦C(P<0.05),while the best studied pH was 9(P<0.05).

The results showed that time factor also had positive effect on biofilm formation , the best biofilm formation occurred after 72 hr.

Key words: adhesion, Bifidobacterium, abiotic surfaces.