Production, extraction and purification of Bacillus licheniformis FH4-IRQ alkaline phosphatase
Other Title(s)
إنتاج و استخلاص و تنقية إنزيم الفوسفاتيز القاعدي من بكتريا Bacillus licheniformis FH4-IRQ
Joint Authors
al-Hilli, Hasan Majid Rashid
Hassan, Shadha Salman
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.219-230, 12 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
The optimum conditions for Bacillus licheniformis FH4-IRQ alkaline phosphatase production on PB ALP medium were determined as :, arabinose 3% , maltose 2% and 3% , cellobiose 1% and 2% , gave high alkaline phosphatase productivity , pH 9 , incubation temperature 45-50 C.
Alkaline phosphatase was produced after 18 hr of inoculation and it increased during the beginning of stationary phase .Alkaline phosphatase was precipitated by ammonium sulphate (60%-95% saturation ) and purified using DEAE Sepharose CL6B ion exchange and Sephacryl S 300 gel filtration , 34.85% of total enzyme was recovered with specific activity 7.066 U/mg and 71.59 folds of purification .
The result refer that the Bacillus licheniformis FH4-IRQ prefer high temperature with alkali conditions to produce alkaline phosphatase without depending on specific carbon source.
American Psychological Association (APA)
al-Hilli, Hasan Majid Rashid& Hassan, Shadha Salman. 2015. Production, extraction and purification of Bacillus licheniformis FH4-IRQ alkaline phosphatase. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.219-230.
https://search.emarefa.net/detail/BIM-955954
Modern Language Association (MLA)
al-Hilli, Hasan Majid Rashid& Hassan, Shadha Salman. Production, extraction and purification of Bacillus licheniformis FH4-IRQ alkaline phosphatase. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.219-230.
https://search.emarefa.net/detail/BIM-955954
American Medical Association (AMA)
al-Hilli, Hasan Majid Rashid& Hassan, Shadha Salman. Production, extraction and purification of Bacillus licheniformis FH4-IRQ alkaline phosphatase. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.219-230.
https://search.emarefa.net/detail/BIM-955954
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 229-230
Record ID
BIM-955954