Production, extraction and purification of Bacillus licheniformis FH4-IRQ alkaline phosphatase

Abstract EN

The optimum conditions for Bacillus licheniformis FH4-IRQ alkaline phosphatase production on PB ALP medium were determined as :, arabinose 3% , maltose 2% and 3% , cellobiose 1% and 2% , gave high alkaline phosphatase productivity , pH 9 , incubation temperature 45-50 C.

Alkaline phosphatase was produced after 18 hr of inoculation and it increased during the beginning of stationary phase .Alkaline phosphatase was precipitated by ammonium sulphate (60%-95% saturation ) and purified using DEAE Sepharose CL6B ion exchange and Sephacryl S 300 gel filtration , 34.85% of total enzyme was recovered with specific activity 7.066 U/mg and 71.59 folds of purification .

The result refer that the Bacillus licheniformis FH4-IRQ prefer high temperature with alkali conditions to produce alkaline phosphatase without depending on specific carbon source.