Detection of some virulence factors of Aeromonas hydrophila isolated from local freshwater fish

Abstract EN

From sixty two bacterial samples obtained from fish of Baghdad local market.

According to the cultural phenotype and biochemical tests, 23 (37.09%) samples were identified as Aeromonas hydrophila and depending final identification confirmed by API 20.

All bacterial strains of Aeromonas.

hydrophila showed a different capacity in hemolytic activity, 12 (52%) strains showed β-hemolytic activity, 9 (39%) isolates were showed α-hemolytic activity and 2 (8.69%) isolates were λ-hemolytic activity.

All isolates produce proteases, lipase DNase with a variable capacity.

Examination susceptibility to commonly used antibiotics referred to the high resistance rate among A.hydrophila strains isolated from local freshwater fish.

We found that all strains enrolled for this study were resistant to ampicillin,Amoxicillin, Cefotaxime while it was fully sensitive to amakacin and azithromycin.

Free cell supernatant of different strains ofAeromonas hydrophyla showed cytotoxic effect on HepG2 cells with different severity