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Identification of ESBL CTX-M-15 genes from isolates of urinary tract infections
Other Title(s)
الكشف عن جينات الأنزيمات المحللة للمضادات الحياتية بيتا لاكتام الواسعة الطيف نوع CTX-M-15 من عزلات التهاب المجاري البولية
Joint Authors
Aziz, Zahid Sadun
al-Jabburi, Salman Aziz Adus
al-Muhanna, Abbas Shakir Jawad
Source
al-Kufa University Journal for Biology
Issue
Vol. 7, Issue 1 (30 Jun. 2015)16 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Abstract EN
The study aimed to evaluate the distribution of CTX-M genes, which coding for ,Extended Spectrum β- lactamases (ESBLs) which hydrolyzed the extended spectrum β- lactams .
Total of 800 urine samples taken from patients suffering from urinary tract infections were collected during the period from February to September from Hospitals in Al-Najaf province.
All samples were cultured on MacConkey agar , were the results revealed that 250 isolates were lactose fermentative versus to 50 isolates were lactose non fermentative.
The isolates succumbed to conventional tests (IMVC), Indol, Methyl red , Voges- Proskauer and Citrate utilization as well as motility test.
Lactose fermentative and non motile isolates were candidate to be confirmed by Vitek2 system.
Initial screening tests by Disk Diffusion test and by MacConkey supplemented with 2 mg / liter of Ceftazidime had been conducted, the resultsof disk diffusion test revealed different resistance phenotypes as well as multi drug resistance patterns Ceftriaxone were found to be the most resistant antibiotics in our isolated bacteria with resistance of 100%.
The next most resistant antibiotics were Ceftazidime (98.11%), followed by aztreonam (90.57%); and cefotaxime (90.27%).
While the result of screening with Ceftazidime reported that 31/53(58.49%) of isolates were initially ESBLs producers.
The results of genotyping tests showed that out of 53 isolates which had been screened for the prevalence of bla CTX-M genes, 47(88.6%) were ositive.
Nucleotide sequence of bla CTX-M of amplicons (19, 20, 22, 23, 24) showed high identity (99%) with CTX-M-15 of NCBI-BLAST.
As concerning with protein sequence, the results showed high identity (98%) with reference protein of gene bank.
The results of other protein sequences revealed great degree of identity reach to (100%) of identity.
American Psychological Association (APA)
Aziz, Zahid Sadun& al-Muhanna, Abbas Shakir Jawad& al-Jabburi, Salman Aziz Adus. 2015. Identification of ESBL CTX-M-15 genes from isolates of urinary tract infections. al-Kufa University Journal for Biology،Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-957637
Modern Language Association (MLA)
Aziz, Zahid Sadun…[et al.]. Identification of ESBL CTX-M-15 genes from isolates of urinary tract infections. al-Kufa University Journal for Biology Vol. 7, no. 1 (2015).
https://search.emarefa.net/detail/BIM-957637
American Medical Association (AMA)
Aziz, Zahid Sadun& al-Muhanna, Abbas Shakir Jawad& al-Jabburi, Salman Aziz Adus. Identification of ESBL CTX-M-15 genes from isolates of urinary tract infections. al-Kufa University Journal for Biology. 2015. Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-957637
Data Type
Journal Articles
Language
English
Notes
Includes appendices.
Record ID
BIM-957637