Assessment of the glucoregulatory enzymes in induced diabetic male rats treated with silver nanoparticles of peel Raphanus sativus L. extract

Abstract EN

This study includes the investigation of the hypoglycemic effect by assay the blood glucose levels and the glucoregulatory enzymes such as Aldolase A, glucose-6-phosphate dehydrogenase, lactate dehydrogenase and Total antioxidant capacity of silver nanoparticles prepared from peel Raphanus sativus L extract in diabetic rats.

The experimental groups (30 rats) were equally divided into five groups six rats for each group: G1 : control group (treated with normal saline) ,G2: diabetic groups induced by alloxan in a single dose (150 mg/kg b.w ),G3: Diabetic groups (alloxan 150 mg/kg b.w intraperitoneal injection for 60 days of 100ppm of Nanoparticles extract,G4: preventive group(intraperitoneal injection for 60 days of 100ppm of Nanoparticles + then induces diabetic by alloxan in a single dose (150mg/kg b.w ),G5: Control treated with 100 ppm of nanoparticles for 60 days.

The results shown a significant decreased in the levels of blood glucose, glucose-6-phosphate dehydrogenase and lactate dehydrogenase for the treated group(G3) and the preventive group(G4) when compared with diabetic group while the Aldolase A activity and total Antioxidant capacity (TOAC) were significantly increased for the treated group(G3) and the preventive group(G4) when compared with diabetic group