Comparison between different methods for detection of salmonella species in imported and local duckling

Abstract EN

Two hundred freshly dead and apparently healthy ducklings from 160 imported and 40 local species (Muller, pekin, Muscovy and Baladi) were bacteriologically examined for isolation and identification of Salmonella species.

Only10 samples were positive with prevalence 5% in all collected samples.

These isolates were further characterized by polymerase chain reaction.

The result revealed 10 different servore as following S.Jedburgh,S.Harrisonburg, S.Braenderup, S.Southbank, S.SekondiII, .Sinchew, S.Brandenburg from imported duckling and S.Ruzizi, S.

Give and S.Entertidis from local duckling with 0.5% for each.

Salmonella isolates were tested for antimicrobial sensitivity and the most resistance rate was for trimethoprim with 80%, intermediate resistance for Penicillin, amoxicillin with 40 % and100% sensitive for norfloxacin.

Based on PCR, all examined salmonella were positive100 %( 7/7) for stn virulence gene, while 42.85% (3/7) of the tested salmonella isolates were positive to aadB antibiotic resistance gene.