Prevalence and molecular detection of some virulence genes among salmonella species isolated from poultry
Other Title(s)
مدى تواجد و الكشف الجزيئي لبعض جينات الضراوة بين أنواع السالمونيلا المعزولة من الدواجن
Joint Authors
Wahdan, Ali
Isawi, Abu al-Khayr Muhammad Ibrahim
al-Sayyid, Mahmud Izzat
al-Sutuhi, Mahmud al-Sayyid
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 24, Issue 2 (31 Dec. 2019), pp.137-145, 9 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The current study aimed to isolate the salmonella species from different organs of broilers with complete phenotypic identification of the recovered isolates.
After that all recovered isolates were tested for detection of some genes responsible for pathogencity.A total of 250 samples from broilers farms were examined bacteriologically for isolation of salmonella.
All recovered isolates were identified phenotypically, biochemically and serologically.
PCR technique was used for detection of invA, hilAand avrA genes.
Bacteriological examination of samples revealed that Salmonella was recovered in 34 samples out of 250 samples (13.6%).
Twelve S.
Typhimurium, 8 S.
Kentucky, 5 S.
Newport, 2 S.
Tamale, 2 S.
Enteritidis, 1 S.
Molade,2 S.
Takoradi, 1 S.
Virchow and 1 S.
Inganda were isolated from broilers with percentage of (35.29%), (23.5%), (14.7%), (5.88%), (5.88%), (2.94%), (5.88%), (2.94%) and (2.94%) respectively.
All tested Salmonella serovars showed positive amplification at 284 bp, 150 bp and 422 bp fragments which specific for the invAgene (common gene), hilAgene and avrA gene respectively, with total percentage (100%) from examined samples.
American Psychological Association (APA)
al-Sayyid, Mahmud Izzat& al-Sutuhi, Mahmud al-Sayyid& Isawi, Abu al-Khayr Muhammad Ibrahim& Wahdan, Ali. 2019. Prevalence and molecular detection of some virulence genes among salmonella species isolated from poultry. Suez Canal Veterinary Medicine Journal،Vol. 24, no. 2, pp.137-145.
https://search.emarefa.net/detail/BIM-958458
Modern Language Association (MLA)
al-Sayyid, Mahmud Izzat…[et al.]. Prevalence and molecular detection of some virulence genes among salmonella species isolated from poultry. Suez Canal Veterinary Medicine Journal Vol. 24, no. 2 (2019), pp.137-145.
https://search.emarefa.net/detail/BIM-958458
American Medical Association (AMA)
al-Sayyid, Mahmud Izzat& al-Sutuhi, Mahmud al-Sayyid& Isawi, Abu al-Khayr Muhammad Ibrahim& Wahdan, Ali. Prevalence and molecular detection of some virulence genes among salmonella species isolated from poultry. Suez Canal Veterinary Medicine Journal. 2019. Vol. 24, no. 2, pp.137-145.
https://search.emarefa.net/detail/BIM-958458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 143-145
Record ID
BIM-958458