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Prevalence and antibiotic sensitivity of mycoplasma spp. isolated from chicken
Joint Authors
Hamzah, M. I.
Dardir, M. A.
Khafaji, A. A. R.
Rahim, M. T.
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 22, Issue 2 (31 Dec. 2017), pp.55-69, 15 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
In order to determine the Prevalance of Mycoplasma isolated from chicken, A total number of 200 samples collected from birds showing respiratory manifestations and apparently healthy chicken of different ages(two weeks: two months) from different localities(Al-ismailia and Sharkeya Governorates).
These samples include 110 samples from respiratory organs(trachea -lung -air sac) ,75 swabs from nasal cleft and 15 samples from fluid of swollen joints.
A trail for isolation and identification of different Mycoplasma was done using conventional and recent techniques.
Primary isolation of the microorganism on PPLO medium, which appeared as fried egg when examined by dissecting microscope yielded118 positive samples with a total incidence rate 59 %.
The highest recovery rate was from respiratory organs (72.7 %) followed by swabs from nasal claft (46.7 %) and samples from swollen joints (20 %).
Application of Digitonin test for differentiation between Mycoplasma and Acholeplasma .
Mycoplasma is digitonin positive while Acholeplasma is digitonin negative.
The incidence of Mycoplasma is 81.3 % and the incidence of Acholeplasma is 18.6 %.
Biochemical characterization of the obtained isolates gave 58 isolates suspected to be M.
gallispectum from different sites of isolation with percentage of 49 % and 18 isolates suspected to be M.gallinarium with percentage of 15.3 % and 3 isolates suspected to be M.synoviae with percentage of 2.5 % and 7 isolates suspected to be M.arginini with percentage of 5.9 %.
Serological identification of isolates using specific antisera was applied which confirmed the presence of M.
gallispectum and M.synoviae but not other genera (M.gallinarium and M.arginini) because of the lack of specific antisera .
The minimal inhibitory concentration (MIC) results cleared that the antimicrobials (Doxycycline was followed by Erythromycin and Tilmicosin) were highly active in inhibition of Mycoplasma in vitro, whereas Streptomycin and Lincospectin and Ciprofloxacin were less effective against the tested isolates.
American Psychological Association (APA)
Khafaji, A. A. R.& Hamzah, M. I.& Dardir, M. A.& Rahim, M. T.. 2017. Prevalence and antibiotic sensitivity of mycoplasma spp. isolated from chicken. Suez Canal Veterinary Medicine Journal،Vol. 22, no. 2, pp.55-69.
https://search.emarefa.net/detail/BIM-958500
Modern Language Association (MLA)
Khafaji, A. A. R.…[et al.]. Prevalence and antibiotic sensitivity of mycoplasma spp. isolated from chicken. Suez Canal Veterinary Medicine Journal Vol. 22, no. 2 (2017), pp.55-69.
https://search.emarefa.net/detail/BIM-958500
American Medical Association (AMA)
Khafaji, A. A. R.& Hamzah, M. I.& Dardir, M. A.& Rahim, M. T.. Prevalence and antibiotic sensitivity of mycoplasma spp. isolated from chicken. Suez Canal Veterinary Medicine Journal. 2017. Vol. 22, no. 2, pp.55-69.
https://search.emarefa.net/detail/BIM-958500
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 65-68
Record ID
BIM-958500