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Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers
Other Title(s)
التوصيف الجزيئي للاشيريشيا كولاي المعزولة من اللبن الخام مع التركيز على عوامل الضراوة و العوامل المقاومة للمضادات الحيوية
Joint Authors
Id, Hamzah Muhammad Ibrahim
Id, Nur al-Huda Abd Allah
Khafaji, Ahmad Ahmad Rifat
Yusuf, Fatimah Muhammad Ahmad
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 22, Issue 2 (31 Dec. 2017), pp.107-120, 14 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In the present study (10) E.coli strains previously isolated from (450) raw milk samples of different sources were analyzed for the determination of their antibiogram and screened for the presence of alkaline phosphatase gene (phoA) and virulence markers for Shiga toxin producing E.coli (STEC); stx1, stx2, and the occurrence of antibiotic resistance genes; nonspecific TEM β-Lactamase (blaTEM),Tetracycline A (tetA), which were investigated by Polymerase chain reaction.
Ten E.coli strains were tested for the antibiotic resistance by using the AST card of the VITEK2.
The antibiotic sensitivity test showed a high resistance towards Ampicillin (100 %), Tetracycline (80 %), Piperacillin(50 %) and Chloramphenicol, Cephaexin and Sulfamethaxole-Trimethoprim (40 %) for each and 30 % of the isolates showed intermediate resistance toward Amoxicillin and Clavulinic acid.
The polymerase chain reaction (PCR) was used to detect the genes encoding for alkaline phosphatase (phoA) , Shiga toxin type 1(stx1),Shiga toxin type 2( stx2) , Nonspecific TEM β-Lactamase (blaTEM),Tetracycline A (tetA).The results showed that all the investigated E.coli strains(n =10) harbored the alkaline phosphatase gene(phoA),100 %.The Shiga toxin type one encoding gene(stx2) was found in one E.coli strain (O158), while none of E.coli strains has the Shiga toxin type 1 gene (stx1).One hundred percentage of the screened E.coli strains carried the blaTEM gene, while 80% of the strains harbored the tetA gene.
American Psychological Association (APA)
Khafaji, Ahmad Ahmad Rifat& Id, Hamzah Muhammad Ibrahim& Yusuf, Fatimah Muhammad Ahmad& Id, Nur al-Huda Abd Allah. 2017. Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers. Suez Canal Veterinary Medicine Journal،Vol. 22, no. 2, pp.107-120.
https://search.emarefa.net/detail/BIM-958509
Modern Language Association (MLA)
Khafaji, Ahmad Ahmad Rifat…[et al.]. Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers. Suez Canal Veterinary Medicine Journal Vol. 22, no. 2 (2017), pp.107-120.
https://search.emarefa.net/detail/BIM-958509
American Medical Association (AMA)
Khafaji, Ahmad Ahmad Rifat& Id, Hamzah Muhammad Ibrahim& Yusuf, Fatimah Muhammad Ahmad& Id, Nur al-Huda Abd Allah. Molecular characterization of Eschericha coli isolated from raw milk samples with emphasis on their antibiotic resistance and virulence markers. Suez Canal Veterinary Medicine Journal. 2017. Vol. 22, no. 2, pp.107-120.
https://search.emarefa.net/detail/BIM-958509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 115-119
Record ID
BIM-958509